College of Horticulture Science and Engineering, Shandong Agricultural University, Taian, 271018, China; Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production in Shandong Taian, 271018, China; Key Laboratory of Biology of Horticultural Crops in Huanghuai Region, Ministry of Agriculture and Rural Affairs, Taian, 271018, China; State Key Laboratory of Crop Biology, Taian, 271018, China.
College of Horticulture Science and Engineering, Shandong Agricultural University, Taian, 271018, China.
Environ Pollut. 2021 Oct 1;286:117321. doi: 10.1016/j.envpol.2021.117321. Epub 2021 May 7.
The negative impact of antibiotic pollution on the agricultural system and human health is a hot issue in the world. However, little information is available on the antibiotics toxicity mechanism and the role of silicon (Si) to alleviate the antibiotics toxicity. In this study, strawberry (Fragaria ananassa) showed excitatory response to low-dose SMZ (1 mg L), but strawberry root and photosynthetic efficiency were damaged under high level. When SMZ level exceeded 10 mg L, H0, O, MDA and relative conductivity increased, while SOD and CAT activities first increased and then decreased. SMZ accumulated more in roots and fruits, but less in stems, and the accumulation increased with the increase of SMZ-dose. Under 1 mg L SMZ, the SMZ accumulation in fruits was 110.54 μg kg, which exceeded the maximum residue limit. SMZ can induce the expression of sul1, sul2 and intI1, and intI1 had the highest abundance. Exogenous application of Si alleviated the toxicity of SMZ, which is mainly related to the degradation of SMZ in soil and the reduction of SMZ absorption by strawberry. In addition, Si relieved root damage, promoted the increase of photosynthetic efficiency, and improved the antioxidant system to resist SMZ toxicity.
抗生素污染对农业系统和人类健康的负面影响是全球关注的热点问题。然而，关于抗生素毒性机制以及硅缓解抗生素毒性的作用的信息却很少。在本研究中，草莓对低剂量磺胺甲恶唑（SMZ，1mg/L）表现出兴奋反应，但在高浓度下草莓的根和光合作用效率受到损害。当 SMZ 浓度超过 10mg/L 时，H0、O、MDA 和相对电导率增加，而 SOD 和 CAT 活性先增加后减少。SMZ 在根部和果实中的积累量较多，在茎部中的积累量较少，且随着 SMZ 剂量的增加而增加。在 1mg/L SMZ 下，果实中 SMZ 的积累量为 110.54μg/kg，超过了最大残留限量。SMZ 可以诱导 sul1、sul2 和 intI1 的表达，其中 intI1 的丰度最高。外源硅的应用缓解了 SMZ 的毒性，这主要与土壤中 SMZ 的降解以及草莓对 SMZ 吸收的减少有关。此外，硅缓解了根的损伤，促进了光合作用效率的提高，并改善了抗氧化系统以抵抗 SMZ 毒性。
