Pennsylvania Cooperative Fish and Wildlife Research Unit, The Pennsylvania State University, 413 Forest Resources Building, University Park, PA, 16802, USA.
Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA, 16802, USA.
Sci Rep. 2021 May 11;11(1):10003. doi: 10.1038/s41598-021-89390-2.
Collection of biological samples for DNA is necessary in a variety of disciplines including disease epidemiology, landscape genetics, and forensics. Quantity and quality of DNA varies depending on the method of collection or media available for collection (e.g., blood, tissue, fecal). Blood is the most common sample collected in vials or on Whatman Flinders Technology Associates (FTA) cards with short- and long-term storage providing adequate DNA for study objectives. The focus of this study was to determine if biological samples stored on Whatman FTA Elute cards were a reasonable alternative to traditional DNA sample collection, storage, and extraction. Tissue, nasal swabs, and ocular fluid were collected from white-tailed deer (Odocoileus virginianus). Tissue samples and nasal swabs acted as a control to compare extraction and DNA suitability for microsatellite analysis for nasal swabs and ocular fluid extracted from FTA Elute cards. We determined that FTA Elute cards improved the extraction time and storage of samples and that nasal swabs and ocular fluid containing pigmented fluid were reasonable alternatives to traditional tissue DNA extractions.
收集生物样本进行 DNA 分析在多个领域都是必要的，包括疾病流行病学、景观遗传学和法医学。收集方法或可用于收集的介质（例如血液、组织、粪便）的不同，会导致 DNA 的数量和质量有所差异。在管或沃特曼弗拉德斯技术协会 (FTA) 卡上收集血液是最常见的样本，短期和长期储存都能为研究目标提供足够的 DNA。本研究的重点是确定沃特曼 FTA Elute 卡上储存的生物样本是否可以替代传统的 DNA 样本收集、储存和提取方法。从白尾鹿（Odocoileus virginianus）中采集组织、鼻腔拭子和眼液。组织样本和鼻腔拭子作为对照，用于比较从 FTA Elute 卡中提取的鼻腔拭子和眼液的提取和微卫星分析的 DNA 适用性。我们确定 FTA Elute 卡可改善样本的提取时间和储存，且含有色素的鼻腔拭子和眼液是替代传统组织 DNA 提取的合理选择。
