Chandrasekhar Srihari Nirguna, Mallikarjun Shanthala B, Salim Henna P
Department of Pediatric and Preventive Dentistry, Coorg Institute of Dental Sciences, Virajpet, Karnataka, India.
Int J Clin Pediatr Dent. 2020 Nov-Dec;13(6):611-616. doi: 10.5005/jp-journals-10005-1838.
To assess the antimicrobial activity of probiotics SK12 and SM18 on and also to compare the antimicrobial activity of SK12 and SM18.
Synthetic strains of were used to study the antimicrobial activity of probiotics SK12 and SM18 using various tests such as disk diffusion, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC). In disk diffusion, the zone of inhibition was measured to assess the antimicrobial activity. Chlorhexidine was used as a control for this test. The MIC and MBC were assessed at different dilutions of the probiotic sample (100 mg/mL, 50 mg/mL, 25 mg/mL, 12.5 mg/mL, 6.25 mg/mL, 3.12 mg/mL, 1.6 mg/mL, 0.8 mg/mL, 0.4 mg/mL, and 0.2 mg/mL).
SM18 demonstrated 20 mm of zone of inhibition, whereas SK12 demonstrated 15 mm showing a less antibacterial activity in comparison to SM18. SM18 was found to be bactericidal and effective at a minimum concentration of 0.8 mg/mL, whereas SK12 was bactericidal and effective at a minimum concentration of 1.6 mg/mL.
Probiotics demonstrate antibacterial activity against cariogenic microflora. SM is 18 having a better antibacterial activity at lower concentrations than SK12 in reducing cariogenic microorganisms. Clinical significance: BLIS K12 and M18 both demonstrated an antibacterial effect on , wherein the use of probiotic in caries prevention is found to be limited. Hence, it is suggestive to reap the bacterial effects of BLIS K12 and M18 in caries prevention.
Chandrasekhar SN, Mallikarjun SB, Salim HP. Comparative Evaluation of Antibacterial Activity of Probiotics SK12 and SM18: An Study. Int J Clin Pediatr Dent 2020;13(6):611-616.
评估益生菌SK12和SM18的抗菌活性，并比较SK12和SM18的抗菌活性。
使用合成菌株，通过多种测试，如纸片扩散法、最低抑菌浓度（MIC）和最低杀菌浓度（MBC），研究益生菌SK12和SM18的抗菌活性。在纸片扩散法中，测量抑菌圈以评估抗菌活性。洗必泰用作该测试的对照。在益生菌样品的不同稀释度（100mg/mL、50mg/mL、25mg/mL、12.5mg/mL、6.25mg/mL、3.12mg/mL、1.6mg/mL、0.8mg/mL、0.4mg/mL和0.2mg/mL）下评估MIC和MBC。
SM18的抑菌圈为20mm，而SK12为15mm，表明与SM18相比，其抗菌活性较低。发现SM18具有杀菌作用，在最低浓度0.8mg/mL时有效，而SK12在最低浓度1.6mg/mL时具有杀菌作用且有效。
益生菌对致龋微生物具有抗菌活性。在降低致龋微生物方面，SM18在较低浓度下比SK12具有更好的抗菌活性。临床意义：BLIS K12和M18均对 表现出抗菌作用，其中发现益生菌在预防龋齿方面的应用有限。因此，建议在预防龋齿中发挥BLIS K12和M18的细菌作用。
Chandrasekhar SN, Mallikarjun SB, Salim HP. 益生菌SK12和SM18抗菌活性的比较评价：一项 研究。《国际临床儿科牙科学杂志》2020；13(6):611 - 616。
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