Millo Grazielle, Juntavee Apa, Ratanathongkam Ariya, Nualkaew Natsajee, Peerapattana Jomjai, Chatchiwiwattana Supaporn
Graduate Student, Department of Pediatric Dentistry, Faculty of Dentistry, Khon Kaen University, Khon Kaen, Thailand.
Associate Professor, Department of Pediatric Dentistry, Faculty of Dentistry, Khon Kaen University, Khon Kaen, Thailand.
Int J Clin Pediatr Dent. 2017 Apr-Jun;10(2):152-157. doi: 10.5005/jp-journals-10005-1426. Epub 2017 Jun 1.
This study evaluated the antibacterial effects of the formulated (PG) gel against and
The PG extract was dissolved in water at 500 mg/mL. High performance liquid chromatography (HPLC) was used for identification and quantification of chemical marker punicalagin. Minimum bactericidal concentration (MBC) and time-kill assay (TKA) were investigated. Antibacterial activities of the formulated PG gel, 2% chlorhexidine (CHX) gel and blank gel were tested by measuring the zones of inhibition through agar well diffusion method.
The HPLC results showed presence of punicalagin at 2023.58 ± 25.29 pg/mL in the aqueous PG extract and at 0.234% (w/w) in the formulated PG gel. The MBC for and were 250, 125, and 500 mg/mL respectively. The TKA of 500 mg/mL aqueous PG extract showed total inhibition of S. and at 6, 1, and 24 hours contact time respectively. Agar well diffusion revealed that for S. CHX gel > PG gel > blank gel; for S. CHX gel = PG gel > blank gel; for CHX gel > PG gel = blank gel. Comparison of the PG gel potency showed that S. = S. >
The PG gel equivalent to 0.234% punicalagin (w/w) inhibited and but not within 24 hours incubation period and has the potential to be used for caries prevention.
Millo G, Juntavee A, Ratanathongkam A, Nualkaew N, Peerapattana J, Chatchiwiwattana S. Antibacterial Inhibitory Effects of Gel on Cariogenic Bacteria: An Study. Int J Clin Pediatr Dent 2017;10(2):152-157.
本研究评估了配制的（PG）凝胶对[具体细菌名称1]和[具体细菌名称2]的抗菌效果。
将PG提取物以500mg/mL的浓度溶解于水中。采用高效液相色谱法（HPLC）对化学标志物石榴皮苷进行鉴定和定量。研究了最低杀菌浓度（MBC）和时间 - 杀菌试验（TKA）。通过琼脂孔扩散法测量抑菌圈，测试配制的PG凝胶、2%氯己定（CHX）凝胶和空白凝胶的抗菌活性。
HPLC结果显示，水相PG提取物中石榴皮苷的含量为2023.58±25.29pg/mL，配制的PG凝胶中为0.234%（w/w）。[具体细菌名称1]、[具体细菌名称2]和[具体细菌名称3]的MBC分别为250、125和500mg/mL。500mg/mL水相PG提取物的TKA显示，在接触时间分别为6、1和24小时时，对[具体细菌名称1]、[具体细菌名称2]和[具体细菌名称3]有完全抑制作用。琼脂孔扩散试验表明，对于[具体细菌名称1]，CHX凝胶>PG凝胶>空白凝胶；对于[具体细菌名称2]，CHX凝胶 = PG凝胶>空白凝胶；对于[具体细菌名称3]，CHX凝胶>PG凝胶 = 空白凝胶。PG凝胶效力的比较显示，[具体细菌名称1]=[具体细菌名称2]>[具体细菌名称3]。
相当于0.234%石榴皮苷（w/w）的PG凝胶在24小时孵育期内对[具体细菌名称1]和[具体细菌名称2]有抑制作用，但对[具体细菌名称3]无抑制作用，具有用于预防龋齿的潜力。
Millo G, Juntavee A, Ratanathongkam A, Nualkaew N, Peerapattana J, Chatchiwiwattana S. Antibacterial Inhibitory Effects of Gel on Cariogenic Bacteria: An Study. Int J Clin Pediatr Dent 2017;10(2):152 - 157.
