Hendi Seyedeh Sareh, Shiri Maryam, Poormoradi Banafsheh, Alikhani Mohammad Yousef, Afshar Saeid, Farmani Abbas
Department of Endodontics, Dental School, Hamadan University of Medical Sciences, Hamadan, Iran.
General Dentist, Hamadan University of Medical Sciences, Hamadan, Iran.
J Lasers Med Sci. 2021 Nov 24;12:e73. doi: 10.34172/jlms.2021.73. eCollection 2021.
The final goal of root canal therapy is to remove of the most bacteria from the root canal. This study aimed at comparing the antibacterial effects of a diode laser with a wavelength of 940nm and silver nanoparticles and the synergic effects of both techniques on . Ninety single-rooted human teeth were decoronated and prepared with rotary files. The samples were irrigated with sodium hypochlorite and EDTA17%. Then they were autoclaved and contaminated with suspension (1.5 × 10 CFU/mL) for 21 days. The samples were fixed in a microtube and were randomly divided into 4 experimental (n=20) groups and a negative control group (n=10) as follows: Group 1: hypochlorite sodium 5%, Group 2: silver nanoparticle, Group 3: diode laser, and Group 4: diode laser and silver nanoparticle. The samples were obtained from dentin chips before and after the intervention. The data were analysed using the Kruskal-Wallis nonparametric test. Furthermore, alterations in bacterial colonies were entered using the Wilcoxon signed ranks test (α=0.05). There was a significant decrease in colony counts for all groups after interventions ( value<0.05). Also, all groups showed more reductions in colony counts compared with the negative control group ( value <0.004). There was a significant reduction for group 1 in comparison with other groups ( value <0.001) and this group had an extreme decrease of colony counts (RCC=100%). There was an important differential between silver nanoparticles and diode laser groups in bacterial counts ( value<0.001) and silver nanoparticles (RCC=83.15%) had more efficiency than the diode laser (RCC=41/33%). RCC of group 4 was 68/52%. Followed by sodium hypochlorite 5%, silver nanoparticles were the most effective antibacterial substances. The 940 nm laser diode had less antibacterial effect compared to its use with silver nanoparticles.
根管治疗的最终目标是清除根管内的大多数细菌。本研究旨在比较波长为940nm的二极管激光和银纳米颗粒的抗菌效果，以及两种技术的协同作用。90颗单根人牙被截冠并用旋转锉进行预备。样本用次氯酸钠和17%的乙二胺四乙酸冲洗。然后进行高压灭菌，并用悬浮液（1.5×10 CFU/mL）污染21天。样本固定在微量管中，随机分为4个实验组（n=20）和1个阴性对照组（n=10），如下：第1组：5%次氯酸钠；第2组：银纳米颗粒；第3组：二极管激光；第4组：二极管激光和银纳米颗粒。在干预前后从牙本质碎屑中获取样本。数据采用Kruskal-Wallis非参数检验进行分析。此外，使用Wilcoxon符号秩检验（α=0.05）输入细菌菌落的变化情况。干预后所有组的菌落计数均显著下降（P值<0.05）。此外，与阴性对照组相比，所有组的菌落计数减少更多（P值<0.004）。与其他组相比，第1组有显著降低（P值<0.001），该组菌落计数极度下降（RCC=100%）。银纳米颗粒组和二极管激光组在细菌计数方面存在重要差异（P值<0.001），银纳米颗粒（RCC=83.15%）比二极管激光（RCC=41/33%）更有效。第4组的RCC为68/52%。仅次于5%次氯酸钠，银纳米颗粒是最有效的抗菌物质。940nm激光二极管与银纳米颗粒联合使用时抗菌效果较差。
