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中国新疆南部儿童中的分子检测。

Molecular detection of in children in southern Xinjiang, China.

作者信息

Zhao Yafei, Ma Wenxuan, Su Duoduo, Zhang Zhenjie, Zhao Aiyun, Yu Fuchang, Qi Meng

机构信息

College of Animal Science and Technology, Tarim University, Alaer 843300, Xinjiang, China.

Engineering Laboratory of Tarim Animal Diseases Diagnosis and Control, Xinjiang Production & Construction Corps, China.

出版信息

Food Waterborne Parasitol. 2025 Jul 4;40:e00275. doi: 10.1016/j.fawpar.2025.e00275. eCollection 2025 Sep.

DOI:10.1016/j.fawpar.2025.e00275
PMID:40688528
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12272601/
Abstract

is a common intestinal parasite in human and animals worldwide. In this study, 609 fecal samples were collected from preschool children in 11 counties in Southern Xinjiang, China. All samples were screened for using PCR targeting rRNA gene, revealing an infection rate of 4.4 % (27/609). Seven of the 11 counties were -positive. The highest infection rate was 15.9 % (10/63) in Yopurga, and the lowest infection rate was 0.9 % (1/109) in Lop. Infection rates in boys and girls were 4.4 % (13/299) and 4.5 % (14/310), respectively. Genetic analysis identified all 27 positive samples as genotype 1. These results confirmed the presence of in children in southern Xinjiang, China. The high degree of sequence homology in the rRNA gene indicates a clonal distribution pattern for .

摘要

是全球人类和动物中常见的肠道寄生虫。在本研究中,从中国新疆南部11个县的学龄前儿童中收集了609份粪便样本。所有样本均使用靶向rRNA基因的PCR进行筛查,感染率为4.4%(27/609)。11个县中有7个县呈阳性。感染率最高的是约普恰克,为15.9%(10/63),最低的是洛浦,为0.9%(1/109)。男孩和女孩的感染率分别为4.4%(13/299)和4.5%(14/310)。基因分析确定所有27个阳性样本均为基因型1。这些结果证实了中国新疆南部儿童中存在。rRNA基因的高度序列同源性表明呈克隆分布模式。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b911/12272601/0092efa3ad4b/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b911/12272601/e0a0cbfe671e/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b911/12272601/0092efa3ad4b/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b911/12272601/e0a0cbfe671e/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b911/12272601/0092efa3ad4b/gr2.jpg

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本文引用的文献

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Dientamoeba fragilis: An emerging pathogen.脆弱双核阿米巴:一种新出现的病原体。
Enferm Infecc Microbiol Clin (Engl Ed). 2025 Apr;43(4):219-226. doi: 10.1016/j.eimce.2025.03.010.
2
The Low Genetic Diversity of Dientamoeba fragilis Isolates in Southwest Turkey and Analysis of Clinical Findings.土耳其西南部脆弱双核阿米巴分离株的低遗传多样性及临床分析。
Acta Parasitol. 2024 Sep;69(3):1576-1586. doi: 10.1007/s11686-024-00896-x. Epub 2024 Aug 20.
3
Molecular detection and genotyping of Dientamoeba fragilis and Blastocystis sp. in housefly Musca domestica (Diptera: Muscidae): first report for Dientamoeba fragilis.
蜚蠊体内脆弱双滴虫和肠内贾第鞭毛虫的分子检测和基因分型:脆弱双滴虫的首次报道。
Parasitol Res. 2024 Apr 16;123(4):183. doi: 10.1007/s00436-024-08202-4.
4
Investigation of intestinal parasites by conventional and molecular methods in children with gastrointestinal system complaints.采用常规和分子方法对有胃肠道系统投诉的儿童进行肠道寄生虫调查。
Parasitol Res. 2023 Jun;122(6):1361-1370. doi: 10.1007/s00436-023-07836-0. Epub 2023 Apr 10.
5
Should Dientamoeba fragillis be looked for in pediatric digestive pathology of an unknown cause? A proposed pilot case-control study.是否应该在病因不明的儿科消化道病理学中寻找脆弱双核阿米巴?一项拟议的病例对照研究。
Enferm Infecc Microbiol Clin (Engl Ed). 2022 Oct;40(8):436-440. doi: 10.1016/j.eimce.2022.03.013.
6
First detection and molecular characterization of Dientamoeba fragilis in cattle.首次在牛体内检测到并对脆弱双核阿米巴进行分子特征分析。
Zoonoses Public Health. 2022 Dec;69(8):897-903. doi: 10.1111/zph.12986. Epub 2022 Jul 7.
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