Leite-Lima Flávia, Bastos Victor Coutinho, Vitório Jéssica Gardone, Duarte-Andrade Filipe Fideles, Pereira Thaís Dos Santos Fontes, Martins-Chaves Roberta Rayra, Cruz Aline Fernanda, de Lacerda Júlio César Tanos, Lebron Yuri Abner Rocha, Moreira Victor Rezende, Santos Lucilaine Valéria de Souza, Lange Liséte Celina, de Macedo Adriana Nori, Diniz Marina Gonçalves, Gomes Carolina Cavaliéri, de Castro Wagner Henriques, Canuto Gisele André Baptista, Gomez Ricardo Santiago
Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Brazil.
Department of Sanitation and Environmental Engineering, School of Engineering, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Brazil.
Oral Dis. 2022 Nov;28(8):2219-2229. doi: 10.1111/odi.13913. Epub 2021 May 26.
We aimed to assess which metabolic pathways would be implicated in the phenotypic changes of the epithelial lining of odontogenic keratocyst after marsupialization, comparing pre- and post-marsupialized lesions with adjacent oral mucosa.
Eighteen formalin-fixed and paraffin-embedded tissues from six subjects were divided into three paired groups: odontogenic keratocyst pre- (n = 6) and post-marsupialization (n = 6), and adjacent oral mucosa (n = 6). The metabolic pathways found in these groups were obtained by high-performance liquid chromatography-mass spectrometry-based untargeted metabolomics performed.
Through putative metabolite annotation followed by pathway enrichment and predictive analysis with automated algorithms (Mummichog and Gene Set Enrichment Analysis), we found differences in many cellular processes that may be involved in inflammation, oxidative stress response, keratinocyte-basal membrane attachment, differentiation, and proliferation functions, all relevant to odontogenic keratocyst pathobiology and the phenotype acquired after marsupialization.
Our study was able to identify several metabolic pathways potentially involved in the metaplastic changes induced by marsupialization of odontogenic keratocysts. An improved comprehension of this process could pave the way for the development of targeted therapies.
我们旨在评估袋形术式后牙源性角化囊肿上皮衬里的表型变化涉及哪些代谢途径，将袋形术式前后的病变与相邻口腔黏膜进行比较。
来自6名受试者的18份福尔马林固定石蜡包埋组织被分为三组配对样本：牙源性角化囊肿术前（n = 6）和术后（n = 6），以及相邻口腔黏膜（n = 6）。通过基于高效液相色谱 - 质谱的非靶向代谢组学分析获得这些组中发现的代谢途径。
通过推测性代谢物注释，随后进行途径富集和使用自动化算法（Mummichog和基因集富集分析）进行预测分析，我们发现在许多可能参与炎症、氧化应激反应、角质形成细胞 - 基底膜附着、分化和增殖功能的细胞过程中存在差异，所有这些都与牙源性角化囊肿的病理生物学以及袋形术式后获得的表型相关。
我们的研究能够识别出可能参与牙源性角化囊肿袋形术式诱导的化生变化的几种代谢途径。对这一过程的更好理解可为靶向治疗的开发铺平道路。
