The State Key Laboratory of Bioreactor Engineering, College of Bioengineering, East China University of Science and Technology, No.130 Meilong Road, Xuhui District, Shanghai 200237, People's Republic of China.
J Agric Food Chem. 2021 May 26;69(20):5618-5627. doi: 10.1021/acs.jafc.1c01109. Epub 2021 May 12.
Natural products with minor side effects have been reported to be an effective adjuvant therapy for glucose and lipid metabolism disorders. Chrysin, a flavone, has a wide range of physiological effects, such as antioxidant, anti-inflammatory, anti-diabetes, anti-hyperlipidemia, and hepatoprotective. This study was designed to explore the effects and mechanism of chrysin on metabolic syndrome using insulin-resistant HepG2 cells and HFD/STZ-induced C57BL/6J mice. The results indicated that chrysin significantly decreased insulin resistance, oxidative stress, inflammation, and liver injury. In addition, chrysin improved glycogen synthesis and fatty acid oxidation and inhibited gluconeogenesis and fatty acid synthesis by regulating GSK3β, G6Paes, PEPCK, SREBP1, FAS, and ACC1. Furthermore, the results of western blot and real-time PCR experiments demonstrated that chrysin modulated glucose and lipid metabolism through the AMPK/PI3K/AKT signaling pathway. Treatment with the AMPK inhibitor verified that AMPK activation is positively correlated with chrysin activity on glycolipid metabolism. This study confirms that chrysin is a potential treatment for glucose and lipid metabolism disorders.
具有较少副作用的天然产物已被报道为治疗葡萄糖和脂质代谢紊乱的有效辅助治疗方法。白杨素是一种黄酮类化合物，具有广泛的生理作用，如抗氧化、抗炎、抗糖尿病、抗高血脂和保肝作用。本研究旨在利用胰岛素抵抗 HepG2 细胞和 HFD/STZ 诱导的 C57BL/6J 小鼠探索白杨素对代谢综合征的影响和作用机制。结果表明，白杨素可显著降低胰岛素抵抗、氧化应激、炎症和肝损伤。此外，白杨素通过调节 GSK3β、G6Paes、PEPCK、SREBP1、FAS 和 ACC1 来改善糖原合成和脂肪酸氧化，并抑制糖异生和脂肪酸合成。此外，Western blot 和实时 PCR 实验结果表明，白杨素通过 AMPK/PI3K/AKT 信号通路调节糖脂代谢。用 AMPK 抑制剂处理证实 AMPK 激活与白杨素对糖脂代谢的活性呈正相关。本研究证实白杨素是治疗葡萄糖和脂质代谢紊乱的一种潜在方法。
