Institute of Inorganic and Analytical Chemistry, University of Münster, 48149 Münster, Germany.
IBE R&D Institute for Lung Health gGmbH, 48149 Münster, Germany.
Metallomics. 2021 Jun 3;13(6). doi: 10.1093/mtomcs/mfab028.
In a dual approach, laser ablation-inductively coupled plasma-mass spectrometry was applied to investigate spleen samples of rats after intratracheal instillation of polyvinylpyrrolidone-coated gold nanoparticles. First, spatially resolved imaging analysis was deployed to investigate gold translocation from the lungs to the spleen and to investigate the distribution pattern of gold in the spleen parenchyma itself. Using the same instrumental setup, laser ablation-inductively coupled plasma-mass spectrometry in single particle mode was applied to determine the species of translocated gold. Single particle analysis allows the determination of particle size distributions and therefore to distinguish between ionic species, intact nanoparticles, and agglomerates. A translocation of instilled gold from the lungs to the spleen was demonstrated for gold nanoparticles of 30 and 50 nm diameter. Furthermore single particle analysis revealed the translocation of intact gold nanoparticles in a non-agglomerated state.
采用双路径法，应用激光烧蚀电感耦合等离子体质谱法研究了气管内滴注聚乙烯吡咯烷酮包覆金纳米粒子后大鼠的脾脏样本。首先，进行空间分辨成像分析以研究金从肺部向脾脏的转移，并研究金在脾脏实质本身中的分布模式。使用相同的仪器设置，应用单颗粒模式的激光烧蚀电感耦合等离子体质谱法来确定转移的金的物种。单颗粒分析可以确定粒径分布，从而区分离子物种、完整的纳米粒子和聚集体。直径为 30 和 50nm 的金纳米粒子从肺部向脾脏的转移得到了证明。此外，单颗粒分析还揭示了完整的非聚集状态的金纳米粒子的转移。
