生长在伊朗的小亚细亚苦艾（Artemisia splendens Willd.）的化学成分、抗菌作用及细胞毒性
Chemical compositions, antimicrobial effects, and cytotoxicity of Asia minor wormwood (Artemisia splendens Willd.) growing in Iran.
作者信息
Heshmati Afshar Fariba, Zadehkamand Masumeh, Rezaei Zahra, Delazar Abbas, Tarhriz Vahideh, Asgharian Parina
机构信息
Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
出版信息
BMC Chem. 2021 May 12;15(1):33. doi: 10.1186/s13065-021-00759-w.
BACKGROUND
Artemisia splendens from the Asteraceae family is a new source of biologically active compounds. The current study investigated to evaluate antimicrobial and cytotoxicity activity of methanolic extracts and their fractions obtained from aerial parts by agar disk diffusion and MTT methods, respectively. The active fractions were subjected to preparative HPLC for isolating the pure compounds, which were structurally elucidated, by H and C NMR.
RESULTS
The results showed that the methanolic extract and its 60% SPE fraction have the anti-proliferative activity on A549 cell line in comparison with the control group. Meanwhile, the methanolic extract and its 40% SPE fraction can inhibit the growth of Gram-positive strains as anti-microbial activity. The 60% SPE fraction also illustrated anti-proliferative activity on the HT-29 cell line compared to the control group. Chromatographic separations via preparative HPLC yielded 5 flavonoids and three flavonoid glycosides.
CONCLUSION
Based on the results it can be concluded that A. splendens as a potential source of cytotoxic and antimicrobial compounds can be used in pharmaceutics.
背景
菊科植物亮蒿是生物活性化合物的新来源。本研究分别通过琼脂扩散法和MTT法，对从地上部分获得的甲醇提取物及其馏分的抗菌和细胞毒性活性进行了评估。对活性馏分进行制备型高效液相色谱以分离纯化合物，并通过氢核磁共振和碳核磁共振对其结构进行了阐明。
结果
结果表明，与对照组相比，甲醇提取物及其60%固相萃取馏分对A549细胞系具有抗增殖活性。同时，甲醇提取物及其40%固相萃取馏分作为抗菌活性可抑制革兰氏阳性菌株的生长。与对照组相比，60%固相萃取馏分对HT-29细胞系也表现出抗增殖活性。通过制备型高效液相色谱进行色谱分离得到了5种黄酮类化合物和3种黄酮苷。
结论
基于这些结果可以得出结论，亮蒿作为细胞毒性和抗菌化合物的潜在来源可用于制药。
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