Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Drug &Food Control, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
BMC Complement Med Ther. 2022 Mar 19;22(1):78. doi: 10.1186/s12906-022-03551-y.
Multi-drug resistant bacteria hazards to the health of humans could be an agent in the destruction of human generation. Natural products of Bacillus species are the main source to access progressive antibiotics that can be a good candidate for the discovery of novel antibiotics. Wild honey as a valuable food has been used in medicine with antimicrobial effects.
Bacillus strains isolated from wild honey were evaluated for the potential antimicrobial activity against human and plant bacterial and fungal pathogens.
Three bacterial isolates were identified as strain Khuz-1 (98.27% similarity with Bacillus safensis subsp. Safensis strain FO-36b), strain Khuz-2 (99.18% similarity with Bacillus rugosus strain SPB7), and strain Khuz-3 (99.78% similarity with Bacillus velezensis strain CR-502) by 16S rRNA gene sequences. The strains were characterized by their ability to inhibit the growth of human and phytopathogenic fungi.
The results indicated that B. rugosus strain Khuz-2 inhibited the growth of phytopathogenic and human fungal more effective than other ones. It seems that the strain Khuz-2 has a suitable antimicrobial and antifungal potential as a good candidate for further pharmaceutical research.
Based on the results of GC-MS, Pyrrolo [1,2-a] pyrazine-1,4-dion, hexahydro-3-(2-methylpropyle) (PPDHM) was the major compound for all strains which have a various pharmacological effect. Isolation and identification of beneficial bacteria from natural sources can play an important role in future pharmaceutical and industrial applications.
多药耐药菌对人类健康的危害可能是人类灭绝的一个因素。芽孢杆菌属的天然产物是获得具有良好候选特性的新型抗生素的主要来源。野生蜂蜜作为一种有价值的食品，具有抗菌作用，已被用于医学。
评估从野生蜂蜜中分离出的芽孢杆菌菌株对人类和植物细菌和真菌病原体的潜在抗菌活性。
通过 16S rRNA 基因序列鉴定出 3 个细菌分离株，分别为 Khuz-1 株（与 Bacillus safensis subsp. Safensis strain FO-36b 的相似度为 98.27%）、Khuz-2 株（与 Bacillus rugosus strain SPB7 的相似度为 99.18%）和 Khuz-3 株（与 Bacillus velezensis strain CR-502 的相似度为 99.78%）。这些菌株的特征是其抑制人类和植物病原真菌生长的能力。
结果表明，B. rugosus 菌株 Khuz-2 比其他菌株更有效地抑制植物病原真菌和人类真菌的生长。看来，该菌株 Khuz-2 具有适当的抗菌和抗真菌潜力，是进一步药物研究的良好候选物。
基于 GC-MS 的结果，吡咯并[1,2-a]吡嗪-1,4-二酮、六氢-3-(2-甲基丙基)（PPDHM）是所有菌株的主要化合物，具有多种药理作用。从天然来源中分离和鉴定有益细菌可以在未来的药物和工业应用中发挥重要作用。
