Mendes Áurea Gabriela Rodrigues, de Sousa Gabriel Gomes Vilar, França Martha de Souza, de Carvalho Carlos Alberto Marques, Batista Evander de Jesus Oliveira, Passos Adelaide da Conceição Fonseca, Oliveira Karen Renata Herculano Matos, Herculano Anderson Manoel, de Moraes Suellen Alessandra Soares
Instituto de Ciências da Saúde, Universidade Federal do Pará, Belém, Pará, Brazil.
Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Pará, Brazil.
Heliyon. 2021 Apr 19;7(4):e06845. doi: 10.1016/j.heliyon.2021.e06845. eCollection 2021 Apr.
Astrocyte reactivity in the spinal cord may occur after peripheral neural damage. However, there is no data to report such reactivity after Achilles tendon injury. We investigate whether changes occur in the spinal cord, mechanical sensitivity and gait in two phases of repair after Achilles tendon injury. Wistar rats were divided into groups: control (CTRL, without rupture), 2 days post-injury (RUP2) and 21 days post-injury (RUP21). Functional and mechanical sensitivity tests were performed at 2 and 21 days post-injury (dpi). The spinal cords were processed, cryosectioned and activated astrocytes were immunostained by GFAP at 21 dpi. Astrocyte reactivity was observed in the L5 segment of the spinal cord with predominance in the white matter regions and decrease in the mechanical threshold of the ipsilateral paw only in RUP2. However, there was gait impairment in both RUP2 and RUP21. We conclude that during the acute phase of Achilles tendon repairment, there was astrocyte reactivity in the spinal cord and impairment of mechanical sensitivity and gait, whereas in the chronic phase only gait remains compromised.
外周神经损伤后，脊髓中的星形胶质细胞可能会发生反应。然而，尚无数据报道跟腱损伤后会出现这种反应。我们研究了跟腱损伤后两个修复阶段脊髓、机械敏感性和步态是否发生变化。将Wistar大鼠分为几组：对照组（CTRL，未破裂）、损伤后2天（RUP2）和损伤后21天（RUP21）。在损伤后2天和21天进行功能和机械敏感性测试。在损伤后21天对脊髓进行处理、冷冻切片，并用GFAP对活化的星形胶质细胞进行免疫染色。仅在RUP2组中，在脊髓L5节段观察到星形胶质细胞反应，以白质区域为主，同侧爪的机械阈值降低。然而，RUP2和RUP21组均存在步态障碍。我们得出结论，在跟腱修复的急性期，脊髓中存在星形胶质细胞反应，机械敏感性和步态受损，而在慢性期，仅步态仍受到影响。
