Hung Yun-Fen, Hsueh Yi-Ping
Institute of Molecular Biology, Academia Sinica, Taipei 11529, Taiwan, ROC.
iScience. 2021 Mar 31;24(4):102384. doi: 10.1016/j.isci.2021.102384. eCollection 2021 Apr 23.
Voluntary exercise is well known to benefit brain performance. In contrast, forced exercise induces inflammation-related stress responses and may cause psychiatric disorders. Here, we unexpectedly found that rotarod testing, a frequently applied assay for evaluating rodent motor coordination, induces anxiety and alters spatial learning/memory performance of mice. Rotarod testing upregulated genes involved in the unfolded protein response and stress responses and downregulated genes associated with neurogenesis and neuronal differentiation. It impacts two downstream pathways. The first is the IL-6-dependent pathway, which mediates rotarod-induced anxiety. The second is the Toll-like receptor 7 (TLR7)-dependent pathway, which is involved in the effect of rotarod exercise on gene expression and its impact on contextual learning and memory of mice. Thus, although rotarod exercise does not induce systemic inflammation, it influences innate immunity-related responses in the brain, controls gene expression and, consequently, regulates anxiety and contextual learning and memory.
众所周知，自愿运动有益于大脑功能。相比之下，强迫运动则会引发与炎症相关的应激反应，并可能导致精神疾病。在此，我们意外地发现，转棒试验（一种常用于评估啮齿动物运动协调性的实验）会诱发小鼠焦虑，并改变其空间学习/记忆能力。转棒试验上调了与未折叠蛋白反应和应激反应相关的基因，同时下调了与神经发生和神经元分化相关的基因。它影响两条下游通路。第一条是白细胞介素-6（IL-6）依赖的通路，该通路介导转棒试验诱发的焦虑。第二条是Toll样受体7（TLR7）依赖的通路，该通路参与转棒运动对基因表达的影响及其对小鼠情境学习和记忆的作用。因此，尽管转棒运动不会诱发全身性炎症，但它会影响大脑中与先天免疫相关的反应，控制基因表达，进而调节焦虑以及情境学习和记忆。
