Yang Huafang, He Peipei, Yin Youcheng, Mao Zhili, Zhang Jing, Zhong Changle, Xie Tian, Wang Anming
College of Materials, Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou, 311121, People's Republic of China.
College of Medicine, Hangzhou Normal University, Hangzhou, 311121, People's Republic of China.
Bioprocess Biosyst Eng. 2021 Oct;44(10):2061-2073. doi: 10.1007/s00449-021-02583-x. Epub 2021 May 13.
To prepare a robust biocatalyst and enhance the removal of bisphenol A in wastewater, succinic anhydride was reacted with laccase to obtain succinic anhydride-modified laccase (SA-laccase) and then co-crystallized with Cu(PO) to form SA-laccase@Cu(PO) hybrid nanoflowers (hNFs). The activity of SA-laccase@Cu(PO) reached 5.27 U/mg, 1.86-, 2.88- and 2.15-fold those of bare laccase@Cu(PO), laccase@Ca(PO) and laccase@epoxy resin, respectively. Compared with free laccase, the obtained hNFs present enhanced activity and tolerance to pH and high temperature in the removal of BPA. Under the optimum conditions of pH 6.0 and 35 °C, BPA removal reached 93.2% using SA-laccase@Cu(PO) hNFs, which was 1.21-fold of that using free laccase. In addition, the obtained SA-laccase@Cu(PO) hNFs retained nearly 90% of their initial catalytic activity for BPA removal after 8 consecutive batch cycles. This efficient method for preparing immobilized laccase can also be further developed and improved to acquire green biocatalysts for removing persistent organic pollutants in wastewater.
为制备一种稳定的生物催化剂并提高废水中双酚A的去除率，使琥珀酸酐与漆酶反应得到琥珀酸酐修饰的漆酶（SA-漆酶），然后与Cu(PO)共结晶形成SA-漆酶@Cu(PO)杂化纳米花（hNFs）。SA-漆酶@Cu(PO)的活性达到5.27 U/mg，分别是裸漆酶@Cu(PO)、漆酶@Ca(PO)和漆酶@环氧树脂活性的1.86倍、2.88倍和2.15倍。与游离漆酶相比，所得hNFs在去除双酚A时表现出更高的活性以及对pH和高温的耐受性。在pH 6.0和35℃的最佳条件下，使用SA-漆酶@Cu(PO) hNFs时双酚A的去除率达到93.2%，是使用游离漆酶时的1.21倍。此外，所得SA-漆酶@Cu(PO) hNFs在连续8个批次循环后仍保留了近90%的初始催化活性用于去除双酚A。这种制备固定化漆酶的有效方法还可以进一步开发和改进，以获得用于去除废水中持久性有机污染物的绿色生物催化剂。
