Mochizuki Chihiro, Nakamura Junna, Nakamura Michihiro
Department of Organ Anatomy & Nanomedicine, Graduate School of Medicine, Yamaguchi University, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505, Japan.
Core Clusters for Research Initiatives of Yamaguchi University, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505, Japan.
ACS Omega. 2022 Aug 12;7(33):29495-29501. doi: 10.1021/acsomega.2c04096. eCollection 2022 Aug 23.
Fetal bovine serum (FBS) particles, which mainly consist of bovine serum albumin, have the potential for biological and medical applications as drug carriers. The coacervation of albumin is a common technique for preparing albumin-based particles. The replacement of salt with novel metal salts such as Cu is an affordable way to embed the metal ion in the albumin-based particles. Further, increased Cu distribution is prevalent in many cancers. Here, we prepared adhesive cell-like FBS-copper phosphate hybrid particles [FBS-Cu(PO)], which exhibited toxicity toward cancer cells, with a narrow size distribution under cell culture conditions for preventing tumor progression. FBS-Cu(PO) showed peroxidase-like activity. In addition, FBS-Cu(PO) was successfully loaded with rhodamine B and conjugated with fluorescein isothiocyanate as models of drugs by coincubation. Thus, we designed a simple preparation method for optimizing FBS-Cu(PO) synthesis under cell culture conditions. FBS-Cu(PO) has significant potential as an efficient reactive oxygen species generator and drug-delivery agent against cancer cells. Furthermore, the RhoB-loaded FBS-Cu(PO) successfully interacted with 4T1 mouse mammary tumor cells and were confirmed to exhibit toxicity.
胎牛血清（FBS）颗粒主要由牛血清白蛋白组成，具有作为药物载体的生物和医学应用潜力。白蛋白的凝聚是制备基于白蛋白的颗粒的常用技术。用新型金属盐如铜替代盐是将金属离子嵌入基于白蛋白的颗粒中的一种经济实惠的方法。此外，铜分布增加在许多癌症中普遍存在。在这里，我们制备了具有粘性细胞样的FBS-磷酸铜混合颗粒[FBS-Cu(PO)]，其在细胞培养条件下对癌细胞表现出毒性，尺寸分布狭窄，可防止肿瘤进展。FBS-Cu(PO)表现出类过氧化物酶活性。此外，通过共孵育，FBS-Cu(PO)成功负载了罗丹明B并与异硫氰酸荧光素偶联作为药物模型。因此，我们设计了一种简单的制备方法，用于在细胞培养条件下优化FBS-Cu(PO)的合成。FBS-Cu(PO)作为一种高效的活性氧生成剂和抗癌细胞的药物递送剂具有巨大潜力。此外，负载RhoB的FBS-Cu(PO)成功与4T1小鼠乳腺肿瘤细胞相互作用，并被证实具有毒性。
