Ex vivo comparison of leak testing of canine jejunal enterotomies: Saline infusion versus air insufflation.

OBJECTIVE

To compare the ability to detect leakage of enterotomy closures by intraluminal injection of saline or air.

STUDY DESIGN

Ex vivo study.

ANIMALS

Grossly normal jejunal segments (n = 60) from five fresh canine cadavers.

METHODS

Eight-centimeter jejunal segments were randomly assigned to two control (saline control [n = 5], air control [5]) and two treatment groups (injection of saline [n = 25] or air [25] after enterotomies were closed in a simple continuous pattern using 4-0 glycomer 631). Initial leak pressure (ILP, mean ± SD), maximum intraluminal pressure (MIP), and leakage location were compared. For all air insufflation constructs, the volume of air insufflated at the time of initial leakage was recorded.

RESULTS

The ILPs of control segments did not differ whether injected with saline (405.71 ± 56.97 mmHg) or air (376.84 ± 42.54, p = 1.00). Enterotomy closures leaked at lower pressures when injected with air (ILP: 68.52 ± 6.56) compared to saline (87.76 ± 5.20, p = .03). Similar results were obtained when comparing MIPs. A moderate association (r = .51) was identified between volume of air infused and ILPs. The strength of the association improved when stratified by cadaver. The location of leakage-most commonly suture tracks-was identified for all air constructs and for 14 of 25 saline constructs.

CONCLUSION

Enterotomy closures leaked at lower pressures after intraluminal injection of air compared to saline.

CLINICAL SIGNIFICANCE

Intraoperative leak testing of small intestinal enterotomy closures may be rendered more sensitive and precise by the use of air instead of saline as the infusate.