Zuraw-Weston Sarah E, Siavashpouri Mahsa, Moustaka Maria E, Gerling Thomas, Dietz Hendrik, Fraden Seth, Ribbe Alexander E, Dinsmore Anthony D
Department of Physics, University of Massachusetts Amherst, Hasbrouck Lab, 666 North Pleasant Street, Amherst, Massachusetts 01002, United States.
Department of Physics, Brandeis University, Abelson-Bass-Yalem, 415 South Street, Waltham, Massachusetts 02454, United States.
Langmuir. 2021 May 25;37(20):6219-6231. doi: 10.1021/acs.langmuir.1c00416. Epub 2021 May 13.
Inspired by the ability of cell membranes to alter their shape in response to bound particles, we report an experimental study of long, slender nanorods binding to lipid bilayer vesicles and altering the membrane shape. Our work illuminates the role of particle concentration, adhesion strength, and membrane tension in determining the membrane morphology. We combined giant unilamellar vesicles with oppositely charged nanorods, carefully tuning the adhesion strength, membrane tension, and particle concentration. With increasing adhesion strength, the primary behaviors observed were membrane deformation, vesicle-vesicle adhesion, and vesicle rupture. These behaviors were observed in well-defined regions in the parameter space with sharp transitions between them. We observed the deformation of the membrane resulting in tubulation, textured surfaces, and small and large lipid-particle aggregates. These responses are robust and repeatable and provide a new physical understanding of the dependence on the shape, binding affinity, and particle concentration in membrane remodeling. The design principles derived from these experiments may lead to new bioinspired membrane-based materials.
受细胞膜响应结合颗粒而改变形状能力的启发，我们报告了一项关于细长纳米棒与脂质双层囊泡结合并改变膜形状的实验研究。我们的工作阐明了颗粒浓度、粘附强度和膜张力在决定膜形态方面的作用。我们将巨型单层囊泡与带相反电荷的纳米棒相结合，仔细调节粘附强度、膜张力和颗粒浓度。随着粘附强度的增加，观察到的主要行为是膜变形、囊泡 - 囊泡粘附和囊泡破裂。这些行为在参数空间的明确区域中观察到，它们之间有明显的转变。我们观察到膜的变形导致形成微管、纹理表面以及大小不一的脂质 - 颗粒聚集体。这些响应是稳健且可重复的，为膜重塑中对形状、结合亲和力和颗粒浓度的依赖性提供了新的物理理解。从这些实验中得出的设计原则可能会导致新型的受生物启发的基于膜的材料。
