Institute of Plant Quarantine Research, Chinese Academy of Inspection and Quarantine, Beijing 100176, PR China.
Canadian Food Inspection Agency (CFIA), Charlottetown Laboratory, Charlottetown, PE C1A 5T1, Canada.
Int J Syst Evol Microbiol. 2021 May;71(5). doi: 10.1099/ijsem.0.004786.
is a Gram-stain-positive bacterium with eight subspecies, five of which have been redefined as different species on the basis of their genome sequence data. On the basis of the results of phylogenetic analysis of gene sequences, strains of members of the genus isolated from barley have been grouped in a separate clade from other species and subspecies of the genus . In this study, the biochemical, physiological, fatty acids and genetic characteristics of strains DM1 and DM3, which represented the barley isolates, were examined. On the basis of results from multi-locus sequence typing and other biochemical and physiological features, including colony colour, carbon source utilisation and enzyme activities, DM1 and DM3 are categorically differentiated from the aforementioned eight species and subspecies of the genus . Moreover, the results of genomic analysis reveal that the DNA G+C contents of DM1 and DM3 are 73.7 and 73.5 %, respectively, and the average nucleotide identity (ANI) values between DM1 and DM3 and other species and subspecies range from 90.4 to 92.0 %. The ANI value between DM1 and DM3 is 98.0 %. These results indicate that DM1 and DM3 are distinct from other known species and subspecies of the genus . Therefore, we propose a novel species, , with DM1 (=CFCC 16553 =LMG 31970) as the type strain.
是一株革兰氏阳性菌，有 8 个亚种，其中 5 个亚种基于其基因组序列数据被重新定义为不同的种。基于基因序列的系统发育分析结果，从大麦中分离出的 属菌株被聚为一个单独的分支，与该属的其他种和亚种分开。在本研究中，对代表大麦分离株的菌株 DM1 和 DM3 的生化、生理、脂肪酸和遗传特性进行了研究。基于多位点序列分型和其他生化和生理特征的结果，包括菌落颜色、碳源利用和酶活性，DM1 和 DM3 与上述 8 个种和亚种有明显的区别。此外，基因组分析的结果表明，DM1 和 DM3 的 DNA G+C 含量分别为 73.7%和 73.5%，DM1 和 DM3 与其他种和亚种之间的平均核苷酸同一性（ANI）值范围为 90.4%至 92.0%。DM1 和 DM3 之间的 ANI 值为 98.0%。这些结果表明，DM1 和 DM3 与其他已知的种和亚种明显不同。因此，我们提出了一个新的种， ，以 DM1（=CFCC 16553=LMG 31970）为模式菌株。
