Program of Food Technology (PPGTA), Federal University of Technology - Paraná (UTFPR-CM), via Rosalina Maria Dos Santos, 1233 CEP 87301-899, Campo Mourão, Paraná, Brazil.
Program of Food Technology (PPGTAL), Federal University of Technology-- Paraná (UTFPR-LD), Avenida dos Pioneiros 3131, CEP 86036-370, Londrina Paraná, Brazil.
Food Chem. 2021 Oct 30;360:129979. doi: 10.1016/j.foodchem.2021.129979. Epub 2021 May 3.
Vitamin D is a water-insoluble compound presented in two main forms (D and D), susceptible to environmental conditions. Microencapsulation is an alternative to supplements and preserve vitamin D properties in foods. Entrapment efficiency (EE) is the main property to evaluate the encapsulation effectiveness and therefore it is of interest the study of analytical methods for the identification and quantification of this compound within the particle. This paper describes a low cost UV-Vis methodology validation to the identification and quantification of vitamin D in microparticles produced by hot homogenization. The method was validated following the International Conference on Harmonization (ICH) guidelines. To guarantee safe application in foodstuff, microparticles toxigenicity was evaluated with Allium cepa L. in vivo model, showing no cytotoxic nor genotoxic potential. High entrapment efficiency was obtained, the results also demonstrated that the concentration of vitamin D in microparticles can be safely accessed by the validated method.
维生素 D 是一种不溶于水的化合物，有两种主要形式（D 和 D），易受环境条件影响。微胶囊化是一种替代补充剂的方法，可以在食品中保留维生素 D 的特性。包封效率（EE）是评估封装效果的主要性质，因此研究用于鉴定和定量分析微胶囊中维生素 D 的分析方法很有意义。本文描述了一种低成本的 UV-Vis 方法，用于鉴定和定量分析通过热均相法制备的微颗粒中的维生素 D。该方法是按照国际协调会议（ICH）的指导原则进行验证的。为了保证在食品中的安全应用，采用洋葱鳞茎体内模型评估了微颗粒的毒性，结果表明没有细胞毒性或遗传毒性。获得了高包封效率，结果还表明，通过验证的方法可以安全地获取微颗粒中维生素 D 的浓度。
