麦糟作为一种可持续且低成本的漆酶固定化载体。
Spent grain as a sustainable and low-cost carrier for laccase immobilization.
机构信息
Department of Chemistry, Sapienza University of Rome, P.le A. Moro 5, 00185 Rome, Italy.
Department of Chemistry, Sapienza University of Rome, P.le A. Moro 5, 00185 Rome, Italy.
出版信息
Waste Manag. 2021 Jun 1;128:114-121. doi: 10.1016/j.wasman.2021.04.055. Epub 2021 May 11.
Spent grain is promising lignocellulosic by-product support for laccase immobilization. The waste digestion with two different approaches (HCl/NaOH and HSO/NaOH) was performed. Different procedures (soaking and dropping), based on chemical and physical reactions, were also used to obtain the highest immobilized activity. Results showed that HSO/NaOH digestion guaranteed an immobilized activity five times higher than HCl/NaOH digestion. The best immobilization conditions with physical dropping procedure resulted in the highest immobilized activity on digested spent grain (2500 U/Kg). Good reusability (42% of activity retained after four cycles), and lower catalytic efficiency (V/K) of 0.053 min than free laccase (0.14 min) with ABTS as substrate, were also obtained. Besides, when 20 mg of biocatalyst (0.02 U) were tested for syringic acid removal, complete oxidation of the phenol was achieved in just 4 h.
酒糟是一种很有前途的木质纤维素副产品，可用于固定化漆酶。采用两种不同方法（HCl/NaOH 和 HSO/NaOH）对其进行了废物消化。还使用了基于化学反应的不同程序（浸泡和滴注）来获得最高的固定化活性。结果表明，HSO/NaOH 消化保证了固定化活性比 HCl/NaOH 消化高五倍。采用物理滴注程序的最佳固定化条件，在消化后的酒糟上获得了最高的固定化活性（2500 U/Kg）。还获得了良好的可重复使用性（经过四个循环后保留了 42%的活性）和较低的催化效率（V/K）（以 ABTS 为底物时为 0.053 min，而游离漆酶为 0.14 min）。此外，当以 20 mg 生物催化剂（0.02 U）测试对丁香酸的去除时，仅 4 小时即可实现酚的完全氧化。
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