Restall Brendon S, Cikaluk Brendyn D, Martell Matthew T, Haven Nathaniel J M, Mittal Rohan, Silverman Sveta, Peiris Lashan, Deschenes Jean, Adam Benjamin A, Kinnaird Adam, Zemp Roger J
University of Alberta, Electrical and Computer Engineering Department, Edmonton, Canada.
Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Canada.
Biomed Opt Express. 2021 Dec 2;13(1):39-47. doi: 10.1364/BOE.443751. eCollection 2022 Jan 1.
A rapid scanning microscopy method for hematoxylin and eosin (H&E) like images is sought after for interoperative diagnosis of solid tumor margins. The rapid observation and diagnosis of histological samples can greatly lower surgical risk and improve patient outcomes from solid tumor resection surgeries. Photoacoustic remote sensing (PARS) has recently been demonstrated to provide images of virtual H&E stains with excellent concordance with true H&E staining of formalin-fixed, paraffin embedded tissues. By using PARS with constant velocity and 1D galvanometer mirror scanning we acquire large virtual H&E images (10mm x 5mm) of prostate tissue in less than 3.5 minutes without staining, and over two orders of magnitude faster data acquisition than the current PARS imaging speed.
人们一直在寻找一种用于获取苏木精和伊红（H&E）样图像的快速扫描显微镜方法，以用于实体肿瘤边缘的术中诊断。对组织学样本进行快速观察和诊断可大大降低手术风险，并改善实体肿瘤切除手术的患者预后。最近已证明，光声遥感（PARS）能够提供虚拟H&E染色图像，与福尔马林固定、石蜡包埋组织的真实H&E染色具有极佳的一致性。通过以恒定速度使用PARS和一维振镜扫描，我们在不到3.5分钟的时间内无需染色即可获取前列腺组织的大型虚拟H&E图像（10mm x 5mm），数据采集速度比当前的PARS成像速度快两个数量级以上。
