Chinese-German Joint Laboratory for Natural Product Research, Key Laboratory of Biological Resources and Ecological Environment of Qinba Areas, School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, China.
Centre of Molecular and Environmental Biology, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal.
Vet Res. 2021 May 13;52(1):70. doi: 10.1186/s13567-021-00943-9.
Antibody mimetics may be used for various biomedical applications, especially those for which conventional antibodies are ineffective. In this study, we developed a smaller molecular chicken IgY mimetic peptide (IgY-peptide) based on the complementarity-determining regions (CDRs) of the anti-canine parvovirus (CPV) IgY-scFv prepared previously. The mimetic peptide showed no cross-reactivity with canine distemper virus (CDV) and canine coronavirus (CCV) and showed excellent protective properties for Crandell-Rees Feline Kidney (CRFK) cells against CPV. This study is the first attempt to develop a mimetic IgY-peptide and demonstrates the ease and feasibility in generating such a novel antibody-like functional molecule for biomedical purposes.
抗体模拟物可用于各种生物医学应用，特别是那些常规抗体无效的应用。在这项研究中，我们基于先前制备的抗犬细小病毒（CPV）IgY-scFv 的互补决定区（CDRs），开发了一种更小的分子鸡 IgY 模拟肽（IgY-肽）。该模拟肽与犬瘟热病毒（CDV）和犬冠状病毒（CCV）无交叉反应性，对猫肾细胞（CRFK）对 CPV 具有优异的保护作用。本研究首次尝试开发模拟 IgY-肽，并证明了针对生物医学目的生成这种新型抗体样功能分子的简便性和可行性。
