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哺乳动物三维基因组结构和组蛋白 H3K9 二甲基化的调控由 H3K9 甲基转移酶完成。

Regulation of mammalian 3D genome organization and histone H3K9 dimethylation by H3K9 methyltransferases.

机构信息

Cellular Memory Laboratory, RIKEN Cluster for Pioneering Research, Wako, Japan.

Laboratory for Developmental Epigenetics, RIKEN Center for Biosystems Dynamics Research, Kobe, Japan.

出版信息

Commun Biol. 2021 May 13;4(1):571. doi: 10.1038/s42003-021-02089-y.

DOI:10.1038/s42003-021-02089-y
PMID:33986449
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8119675/
Abstract

Histone H3 lysine 9 dimethylation (H3K9me2) is a highly conserved silencing epigenetic mark. Chromatin marked with H3K9me2 forms large domains in mammalian cells and overlaps well with lamina-associated domains and the B compartment defined by Hi-C. However, the role of H3K9me2 in 3-dimensional (3D) genome organization remains unclear. Here, we investigated genome-wide H3K9me2 distribution, transcriptome, and 3D genome organization in mouse embryonic stem cells following the inhibition or depletion of H3K9 methyltransferases (MTases): G9a, GLP, SETDB1, SUV39H1, and SUV39H2. We show that H3K9me2 is regulated by all five MTases; however, H3K9me2 and transcription in the A and B compartments are regulated by different MTases. H3K9me2 in the A compartments is primarily regulated by G9a/GLP and SETDB1, while H3K9me2 in the B compartments is regulated by all five MTases. Furthermore, decreased H3K9me2 correlates with changes to more active compartmental state that accompanied transcriptional activation. Thus, H3K9me2 contributes to inactive compartment setting.

摘要

组蛋白 H3 赖氨酸 9 二甲基化 (H3K9me2) 是一种高度保守的沉默表观遗传标记。在哺乳动物细胞中，用 H3K9me2 标记的染色质形成大域，并且与核纤层相关域和由 Hi-C 定义的 B 区室很好地重叠。然而，H3K9me2 在三维 (3D) 基因组组织中的作用尚不清楚。在这里，我们在抑制或耗尽 H3K9 甲基转移酶 (MTases)：G9a、GLP、SETDB1、SUV39H1 和 SUV39H2 后，研究了小鼠胚胎干细胞中全基因组 H3K9me2 分布、转录组和 3D 基因组组织。我们表明，H3K9me2 受所有五个 MTases 调控；然而，A 和 B 区室中的 H3K9me2 和转录受不同的 MTases 调控。A 区室中的 H3K9me2 主要由 G9a/GLP 和 SETDB1 调控，而 B 区室中的 H3K9me2 则由所有五个 MTases 调控。此外，H3K9me2 的减少与更活跃的区室状态变化相关，这种状态变化伴随着转录激活。因此，H3K9me2 有助于建立非活性区室。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e79/8119675/192da26b5a17/42003_2021_2089_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e79/8119675/7f263bb66bbd/42003_2021_2089_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e79/8119675/f72f139559a2/42003_2021_2089_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e79/8119675/8fb6b16593c3/42003_2021_2089_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e79/8119675/3d8a61de3e22/42003_2021_2089_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e79/8119675/56d5eda42eed/42003_2021_2089_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e79/8119675/192da26b5a17/42003_2021_2089_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e79/8119675/7f263bb66bbd/42003_2021_2089_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e79/8119675/f72f139559a2/42003_2021_2089_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e79/8119675/8fb6b16593c3/42003_2021_2089_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e79/8119675/3d8a61de3e22/42003_2021_2089_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e79/8119675/56d5eda42eed/42003_2021_2089_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e79/8119675/192da26b5a17/42003_2021_2089_Fig6_HTML.jpg

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2
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3
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5
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