Choudhry Hani, Hassan Mohammed A, Al-Malki Abdulrahman L, Al-Sakkaf Kaltoom A
Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah 21589, Kingdom of Saudi Arabia.
Cancer and Mutagenesis Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Kingdom of Saudi Arabia.
Oncol Lett. 2021 Jul;22(1):508. doi: 10.3892/ol.2021.12769. Epub 2021 May 3.
Long non-coding RNAs (lncRNAs), a type of cellular RNA, play a critical regulatory role in several physiological developments and pathological processes, such as tumorigenesis and tumor progression. Obesity is a risk factor for a number of serious health conditions, including breast cancer (BC). However, the underlying mechanisms behind the association between obesity and increased BC incidence and mortality remain unclear. Several studies have reported changes in lncRNA expression due to obesity and BC, independently encouraging further investigation of the relationship between the two in connection with lncRNAs. The present study was designed to first screen for the expression of 29 selected lncRNAs that showed a link to cancer or obesity in the blood of a selected cohort of 6 obese and 6 non-obese patients with BC. The expression levels of significantly expressed lncRNAs, and , were further investigated in a larger cohort of 69 patients with BC (36 obese and 33 non-obese), using reverse transcription-quantitative polymerase chain reaction. Results showed not only that remained significantly downregulated in the larger cohort (P=0.002), but also that it was associated with several clinicopathological characteristics, such as negative HER2 status, negative E-cadherin expression, negative vascular invasion, negative margin invasion and LCIS. These findings suggest that obesity may have a role in inhibiting expression, which may serve as a novel potential biomarker and therapeutic target for BC.
长链非编码RNA（lncRNAs）是一种细胞RNA，在多种生理发育和病理过程中发挥关键调节作用，如肿瘤发生和肿瘤进展。肥胖是包括乳腺癌（BC）在内的多种严重健康问题的危险因素。然而，肥胖与BC发病率和死亡率增加之间关联的潜在机制仍不清楚。几项研究报告了由于肥胖和BC导致的lncRNA表达变化，这独立地鼓励进一步研究两者与lncRNAs之间的关系。本研究旨在首先筛选29种选定的lncRNAs在6名肥胖和6名非肥胖BC患者的选定队列血液中的表达，这些lncRNAs与癌症或肥胖有关。使用逆转录定量聚合酶链反应在69名BC患者（36名肥胖和33名非肥胖）的更大队列中进一步研究显著表达的lncRNAs 和 的表达水平。结果不仅显示 在更大队列中仍显著下调（P = 0.002），而且它与几种临床病理特征相关，如HER2状态阴性、E-钙黏蛋白表达阴性、血管侵犯阴性、切缘侵犯阴性和小叶原位癌。这些发现表明，肥胖可能在抑制 表达中起作用，这可能作为BC的一种新的潜在生物标志物和治疗靶点。
