受砷污染的饮用水对蚕豆姐妹染色单体交换的诱导作用。

Induction of sister-chromatid exchanges in Vicia faba by arsenic-contaminated drinking water.

作者信息

Gómez-Arroyo S, Hernández-García A, Villalobos-Pietrini R

机构信息

Laboratorio de Citogenética y Mutagénesis Ambientales, Universidad Nacional Autónoma de México, D.F., México.

出版信息

Mutat Res. 1988 Jul;208(3-4):219-24. doi: 10.1016/0165-7992(88)90064-4.

DOI:10.1016/0165-7992(88)90064-4

PMID:3398872

Abstract

Arsenic-contaminated drinking water from various towns of Comarca Lagunera, Coahuila, Mexico, was tested for its ability to induce sister-chromatid exchanges (SCE) in Vicia faba. 3-h treatments were applied and the differential staining technique of Tempelaar et al. (1982) was used. Atomic absorption spectrophotometry showed that the arsenic concentration in drinking water was 0.11-0.695 ppm, well over the maximum limit of 0.05 ppm (EPA, 1984). In all cases the SCE frequencies were significantly different from the controls. Some concentrations (0.2, 0.3, 0.5 and 1.0 ppm) of sodium arsenate (V) and potassium arsenite (III) were also applied to Vicia faba and all produced significant SCE frequencies, except 0.2 ppm of sodium arsenate.

摘要

对墨西哥科阿韦拉州拉古内拉地区各城镇受砷污染的饮用水进行了测试，以检测其在蚕豆中诱导姐妹染色单体交换（SCE）的能力。采用了3小时的处理，并使用了Tempelaar等人（1982年）的差异染色技术。原子吸收分光光度法显示，饮用水中的砷浓度为0.11 - 0.695 ppm，远超过0.05 ppm的最大限值（美国环境保护局，1984年）。在所有情况下，SCE频率与对照组均有显著差异。还将一些浓度（0.2、0.3、0.5和1.0 ppm）的砷酸钠（V）和亚砷酸钾（III）应用于蚕豆，除了0.2 ppm的砷酸钠外，所有处理均产生了显著的SCE频率。