Laboratory of Molecular Biology - Faculty of Pharmacy and Biochemistry - National University of San Marcos, Lima 1, Peru; Laboratory of Toxicological Biochemistry - Biochemistry Department - Faculty of Pharmacy and Biochemistry - National University of San Marcos, Lima 1, Peru.
Laboratory of Molecular Biology - Faculty of Pharmacy and Biochemistry - National University of San Marcos, Lima 1, Peru.
Food Chem. 2021 Oct 30;360:129951. doi: 10.1016/j.foodchem.2021.129951. Epub 2021 Apr 24.
The kanihua (Chenopodium pallidicaule Aellen) Andean grain from the Peruvian Altiplano presents proteins of 15% to 19%. The objective was to obtain purified bioactive antimicrobial peptides (AMPs), hydrolyzed with Alcalase and Pepsin-pancreatin sequential system of protein fractions of kanihua varieties Ramis (KR) and Cupi-Sayhua (KS), and hydrolysates with different degrees of hydrolysis (DH) and percentage inhibition (IP) of the growth of E. coli, S. aureus, and C. albicans. To obtain AMPs, nutraceuticals, bio-preservatives, and novel ingredients in food design. The results showed 216 hydrolysates (1%, w/v), only 28 presented significant difference compared to controls (IP ≥ 45%, p ≤ 0.05), 4 AMPs were purified by chromatography, glutelins KS 4 h (1:10) stood out with DH 40% and IP 52% and 70% of S. aureus and C. albicans, respectively (p ≤ 0.05), showed minimum inhibitory concentration (MIC) of 95% for E. coli (p ≤ 0.05), and presented an anionic charge. In conclusion, the simulated digestion in vitro showed higher DH (7%-67%) than Alcalase (13%-54%); the majority were extensive; of 28 hydrolysates with IP ≥ 45% 4 AMPs with important IPs were obtained, and one was anionic.
安第斯谷物卡尼瓦（Chenopodium pallidicaule Aellen）来自秘鲁高原，其蛋白质含量为 15%至 19%。本研究旨在获得纯化的生物活性抗菌肽（AMPs），为此使用 Alcalase 和胃蛋白酶-胰酶顺序系统水解卡尼瓦品种 Ramis（KR）和 Cupi-Sayhua（KS）的蛋白质级分，并对不同水解度（DH）和对大肠杆菌、金黄色葡萄球菌和白色念珠菌生长抑制率（IP）的水解产物进行分析。目的是获得 AMPs、营养保健品、生物防腐剂和新型食品设计成分。结果表明，在 216 种 1%（w/v）的水解产物中，仅有 28 种与对照组相比具有显著差异（IP≥45%，p≤0.05），4 种 AMP 通过色谱法进行了纯化，KS 4 h 谷蛋白（1:10）表现出 40%的 DH 和 52%、70%的金黄色葡萄球菌和白色念珠菌的 IP，（p≤0.05），对大肠杆菌的最小抑菌浓度（MIC）为 95%（p≤0.05），并呈现出阴离子电荷。总之，体外模拟消化显示出比 Alcalase 更高的 DH（7%-67%）；多数为广泛水解；在 28 种 IP≥45%的水解产物中，获得了 4 种具有重要 IP 的 AMP，其中 1 种为阴离子。
