Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052, Ghent, Belgium.
VIB Center for Plant Systems Biology, B-9052, Ghent, Belgium.
Nat Commun. 2021 May 14;12(1):2842. doi: 10.1038/s41467-021-23112-0.
Plants respond to mild warm temperature conditions by increased elongation growth of organs to enhance cooling capacity, in a process called thermomorphogenesis. To this date, the regulation of thermomorphogenesis has been exclusively shown to intersect with light signalling pathways. To identify regulators of thermomorphogenesis that are conserved in flowering plants, we map changes in protein phosphorylation in both dicots and monocots exposed to warm temperature. We identify MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE KINASE4 (MAP4K4)/TARGET OF TEMPERATURE3 (TOT3) as a regulator of thermomorphogenesis that impinges on brassinosteroid signalling in Arabidopsis thaliana. In addition, we show that TOT3 plays a role in thermal response in wheat, a monocot crop. Altogether, the conserved thermal regulation by TOT3 expands our knowledge of thermomorphogenesis beyond the well-studied pathways and can contribute to ensuring food security under a changing climate.
植物通过器官的伸长生长来应对温和的温暖温度条件，以增强冷却能力，这一过程称为热形态发生。迄今为止，热形态发生的调节已被证明与光信号通路完全交叉。为了鉴定在开花植物中保守的热形态发生调节剂，我们在暴露于温暖温度的双子叶植物和单子叶植物中绘制了蛋白质磷酸化变化的图谱。我们确定丝裂原活化蛋白激酶激酶激酶激酶 4（MAP4K4）/温度目标 3（TOT3）作为热形态发生调节剂，它影响拟南芥中的油菜素甾体信号。此外，我们还表明 TOT3 在小麦（单子叶作物）中的热反应中发挥作用。总的来说，TOT3 的保守热调节扩展了我们对热形态发生的认识，超越了研究充分的途径，并有助于在气候变化下确保粮食安全。
