College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
Center for Translational Microbiome Research, Department of Molecular, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden.
NPJ Biofilms Microbiomes. 2021 May 14;7(1):45. doi: 10.1038/s41522-021-00215-6.
Ruminal microbiota changes frequently with high grain diets and the occurrence of subacute ruminal acidosis (SARA). A grain-induced goat model of SARA, with durations of a significant decrease in the rumen pH value to less than 5.6 and an increase in the rumen lipopolysaccharides concentration, is constructed for real-time monitoring of bacteria alteration. Using 16 S rRNA gene sequencing, significant bacterial differences between goats from the SARA and healthy groups are identified at every hour for six continuous hours after feeding. Moreover, 29 common differential genera between two groups over 6 h after feeding are all related to the altered pH and lipopolysaccharides. Transplanting the microbiota from donor goats with SARA could induce colonic inflammation in antibiotic-pretreated mice. Overall, significant differences in the bacterial community and rumen fermentation pattern between the healthy and SARA dairy goats are real-time monitored, and then tested using ruminal microbe transplantation to antibiotic-treated mice.
瘤胃微生物群落随高精谷物日粮和亚急性瘤胃酸中毒（SARA）的发生而频繁变化。为了实时监测细菌变化，构建了一个以高精谷物为食的 SARA 山羊模型，其特征是瘤胃 pH 值显著下降至低于 5.6，同时瘤胃内脂多糖浓度升高。通过 16S rRNA 基因测序，在喂食后连续 6 小时的每小时，都可以发现 SARA 组和健康组山羊之间存在显著的细菌差异。此外，在喂食后 6 小时内，两组之间的 29 个共同差异属都与 pH 值和脂多糖的变化有关。将 SARA 供体山羊的微生物群移植到抗生素预处理的小鼠中可诱导其结肠炎症。总之，实时监测了健康和 SARA 奶牛山羊之间的细菌群落和瘤胃发酵模式的显著差异，并使用瘤胃微生物移植到抗生素处理的小鼠中进行了测试。
