School of Pharmacy, Minzu University of China, Key Laboratory of Ethnomedicine (Minzu University of China), Ministry of Education, Beijing, 100081, China.
J Microbiol. 2021 Jul;59(7):693-701. doi: 10.1007/s12275-021-0454-8. Epub 2021 May 15.
Due to the different rates of diabetes in different ethnic groups and the structural differences in intestinal microbiota, this study evaluated the changes in diabetes-related intestinal microbiota in two ethnic groups. Fifty-six stool samples were collected from subjects from the Han and Mongolian ethnic groups in China, including participants without diabetes (non-diabetic, ND) and with type 2 diabetes (T2D). The 16S rDNA gene V3 + V4 area was extracted from microbiota, amplified by PCR, and used to perform high-throughput sequencing and screen differential microbiota associated with ethnicity. The results showed that there were 44 T2D-related bacterial markers in the Han subjects, of which Flavonifractor, Alistipes, Prevotella, Oscillibacter, Clostridium XlVa, and Lachnospiracea_incertae_sedis were most closely related to diabetes. There were 20 T2D-related bacterial markers in the Mongolian subjects, of which Fastidiosipila and Barnesiella were most closely related to diabetes. The common markers of T2D bacteria in the two ethnic groups were Papillibacter and Bifidobacterium. There were 17 metabolic pathways with significant differences between the ND and T2D groups in the Han group, and 29 metabolic pathways in the Mongolian group. The glutamatergic metabolic pathway was the only common metabolic pathway in two ethnic groups. The composition and function of diabetes-related bacteria were significantly different among the different ethnic groups, which suggested that the influence of ethnic differences should be fully considered when studying the association between diabetes and bacteria. In addition, the common bacterial markers found in diabetic patients of different ethnic groups in this study can be used as potential targets to study the pathogenesis and treatment of diabetes.
由于不同民族糖尿病发病率不同，肠道微生物结构存在差异，本研究评估了两个民族糖尿病相关肠道微生物的变化。共采集中国汉族和蒙古族人群 56 例粪便样本，包括无糖尿病（非糖尿病，ND）和 2 型糖尿病（T2D）参与者。从微生物中提取 16S rDNA 基因 V3+V4 区，经 PCR 扩增后进行高通量测序，筛选与民族相关的差异微生物。结果表明，汉族 T2D 相关细菌标志物有 44 个，其中 Flavonifractor、Alistipes、Prevotella、Oscillibacter、Clostridium XlVa 和 Lachnospiracea_incertae_sedis 与糖尿病的关系最为密切。蒙古族 T2D 相关细菌标志物有 20 个，其中 Fastidiosipila 和 Barnesiella 与糖尿病的关系最为密切。两组共有的 T2D 细菌标志物为 Papillibacter 和 Bifidobacterium。汉族 ND 与 T2D 组间有 17 条代谢途径存在显著差异，蒙古族组有 29 条代谢途径。两组共有的谷氨酸代谢途径是唯一的共同代谢途径。不同民族间糖尿病相关细菌的组成和功能存在显著差异，提示在研究糖尿病与细菌的相关性时，应充分考虑民族差异的影响。此外，本研究发现不同民族糖尿病患者共有细菌标志物可作为研究糖尿病发病机制和治疗的潜在靶点。
