Wang Ye, Luo Xin, Mao Xinmin, Tao Yicun, Ran Xinjian, Zhao Haixia, Xiong Jianhui, Li Linlin
Pharmacological Department, Basic Medicine College, Xinjiang Medical University, Urumqi, Xinjiang, China.
Pharmaceutical Preparation Department, the First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
PLoS One. 2017 Mar 22;12(3):e0172774. doi: 10.1371/journal.pone.0172774. eCollection 2017.
The gut microbiome may have an important influence on the development of diabetes mellitus type 2 (DM2). To better understand the DM2 pandemic in ethnic minority groups in China, we investigated and compared the composition and richness of the gut microbiota of healthy, normal glucose tolerant (NGT) individuals and DM2 patients from two ethnic minority groups in Xinjiang, northwest China, the Uygurs and Kazaks. The conserved V6 region of the 16S rRNA gene was amplified by PCR from the isolated DNA. The amplified DNA was sequenced and analyzed. An average of 4047 high quality reads of unique tag sequences were obtained from the 40 Uygurs and Kazaks. The 3 most dominant bacterial families among all participants, both healthy and DM2 patients, were the Ruminococcaceae, Lachnospiraceae, and Enterobacteriaceae. Significant differences in intestinal microbiota were found between the NGT individuals and DM2 patients, as well as between the two ethnic groups. Our findings shed new light on the gut microbiome in relation to DM2. The differentiated microbiota data may be used for potential biomarkers for DM2 diagnosis and prevention.
肠道微生物群可能对2型糖尿病（DM2）的发展具有重要影响。为了更好地了解中国少数民族中的DM2流行情况，我们调查并比较了来自中国西北部新疆两个少数民族——维吾尔族和哈萨克族的健康、糖耐量正常（NGT）个体及DM2患者的肠道微生物群的组成和丰富度。通过PCR从分离的DNA中扩增16S rRNA基因的保守V6区域。对扩增的DNA进行测序和分析。从40名维吾尔族和哈萨克族个体中平均获得了4047个高质量的独特标签序列读数。在所有参与者（包括健康个体和DM2患者）中，3个最主要的细菌家族是瘤胃球菌科、毛螺菌科和肠杆菌科。在NGT个体与DM2患者之间以及两个民族之间均发现了肠道微生物群的显著差异。我们的研究结果为与DM2相关的肠道微生物群提供了新的见解。这些有差异的微生物群数据可能用作DM2诊断和预防的潜在生物标志物。
