产气克雷伯菌在连续培养条件下生长时己糖激酶和葡萄糖-磷酸烯醇式丙酮酸磷酸转移酶的合成
Hexokinase and glucose-phosphoenolpyruvate phosphotransferase synthesis in Klebsiella aerogenes strains growing in continuous culture.
作者信息
Carter I S, Dean A C
出版信息
Biochem J. 1977 Sep 15;166(3):643-6. doi: 10.1042/bj1660643.
Abstract
Considerable differences in steady-state hexokinase specific activity were found in 16 N.C.I.B. strains of Klebsiella aerogenes grown in identical conditions in glucose-limited chemostats. Strains of N.C.I.B. 8258 had no detectable activity, but its glucose-phosphoenolpyruvate phosphotransferase specific activity and that of the other strains were closely similar, and it is concluded that this phosphotransferase activity regulates the overall utilization of glucose, in which hexokinase plays no essential role. The hexokinase activity was subject to regulation by the availability of phosphorus, but this did not affect the glucose phosphotransferase activity. tlactose-grown organisms (including strain N.C.I.B. 8258) had no glucose phosphotransferase activity, but more than adequate hexokinase activity to phosphorylate the intracellularly liberated glucose.
摘要
在相同条件下于葡萄糖限制的恒化器中培养的16株产气克雷伯菌N.C.I.B.菌株中，发现其稳态己糖激酶比活性存在显著差异。N.C.I.B. 8258菌株没有可检测到的活性，但其葡萄糖 - 磷酸烯醇式丙酮酸磷酸转移酶比活性与其他菌株的非常相似，由此得出结论，这种磷酸转移酶活性调节葡萄糖的整体利用，而己糖激酶在其中不发挥重要作用。己糖激酶活性受磷可用性的调节，但这并不影响葡萄糖磷酸转移酶活性。以乳糖生长的生物体（包括N.C.I.B. 8258菌株）没有葡萄糖磷酸转移酶活性，但具有足够的己糖激酶活性来磷酸化细胞内释放的葡萄糖。
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