Neijssel O M, Hardy G P, Lansbergen J C, Tempest D W, O'Brien R W
Arch Microbiol. 1980 Mar;125(1-2):175-9. doi: 10.1007/BF00403216.
A consistent difference was found between glucose-limited cultures of Escherichia coli and Klebsiella aerogenes strains in the manner which their apparent cellular content of glucose:phosphoenolpyruvate phosphotransferase (glucose-PTS) varied with growth rate. With the former strains, activity increased as a function of growth rate; in the latter it decreased. However, under glucose-sufficient conditions (potassium- or ammonia-limitation) both species behaved similarly; the glucose-PTS activity was lower and bore no obvious relationship to the rate of glucose consumption expressed by the growing culture. These results are discussed in relation to the role of glucose as a regulator of glucose-PTS synthesis, and to the likely contribution which the glucose-PTS makes to the overall rate of glucose uptake, particularly by cells gorwing in glucose-sufficient environments.
在葡萄糖受限的大肠杆菌和产气克雷伯菌培养物中，发现它们的葡萄糖：磷酸烯醇式丙酮酸磷酸转移酶（葡萄糖-PTS）的表观细胞含量随生长速率变化的方式存在一致差异。对于前一种菌株，活性随生长速率增加；而对于后一种菌株，活性则降低。然而，在葡萄糖充足的条件下（钾或氨受限），两种菌的表现相似；葡萄糖-PTS活性较低，且与生长培养物的葡萄糖消耗速率没有明显关系。本文讨论了这些结果与葡萄糖作为葡萄糖-PTS合成调节剂的作用，以及葡萄糖-PTS对葡萄糖总体摄取速率的可能贡献，特别是对在葡萄糖充足环境中生长的细胞的贡献。
