Hunter I S, Kornberg H L
Biochem J. 1979 Jan 15;178(1):97-101. doi: 10.1042/bj1780097.
Dilute cultures of wild-type Escherichia coli K12 and of derivatives impaired in one or other Enzyme-II component of the glucose phosphotransferase system were grown in continuous culture under glucose limitation. Cells harvested from the chemostat took up [U-14C]glucose from 0.1 mM solutions at rates directly related to the rates at which those cells had grown; the activity of the phosphotransferase system in those cells, rendered permeable with optimal accounts of toluene, parallels the ability of the cells to take up glucose. The capacity of these systems was rate-limiting for growth under the negligibly low glucose concentration in the chemostat, but was adequate to account for the stimulation of respiration observed when the cells were presented suddenly with excess glucose.
野生型大肠杆菌K12及其葡萄糖磷酸转移酶系统中一种或其他酶II组分受损的衍生物的稀释培养物，在葡萄糖限制条件下进行连续培养。从恒化器收获的细胞以与这些细胞生长速率直接相关的速率从0.1 mM溶液中摄取[U-14C]葡萄糖；这些细胞中的磷酸转移酶系统活性，在用最佳量的甲苯使其通透后，与细胞摄取葡萄糖的能力平行。在恒化器中极低的葡萄糖浓度下，这些系统的能力是生长的限速因素，但足以解释当细胞突然接触过量葡萄糖时观察到的呼吸刺激现象。
