McLennan A G, Keir H M
Biochem Soc Symp. 1977(42):55-73.
DNA-dependent DNA polymerases have now been purified from a number of invertebrate animals, protists, higher plants and fungi. In this article we review the properties of these enzymes and compare them with the better-known enzymes of vertebrate animals and prokaryotes. Three facts emerge. Firstly, plants, protists and fungi contain high-molecular-weight DNA polymerases which may be capable of categorization into two groups on the basis of their properties in vitro. Secondly, no enzyme analogous to the vertebrate polymerase-beta has yet been found in such organisms, and thirdly, many of these enzymes possess associated exonuclease activities like those of the bacterial DNA polymerases. On the basis of these findings, some tentative proposals are made about the evolution of DNA polymerases.
如今，已从多种无脊椎动物、原生生物、高等植物和真菌中纯化出了依赖DNA的DNA聚合酶。在本文中，我们综述了这些酶的特性，并将它们与脊椎动物和原核生物中更为人熟知的酶进行比较。有三个事实凸显出来。其一，植物、原生生物和真菌含有高分子量的DNA聚合酶，根据它们的体外特性，这些酶可能能够分为两类。其二，在这些生物体中尚未发现类似于脊椎动物聚合酶β的酶，其三，这些酶中的许多都具有与细菌DNA聚合酶类似的相关核酸外切酶活性。基于这些发现，我们对DNA聚合酶的进化提出了一些初步建议。
