SCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Environmental Theoretical Chemistry, South China Normal University, Guangzhou 510006, China; School of Environment, South China Normal University, Guangzhou 510006, China.
Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
Sci Total Environ. 2021 Sep 15;787:147582. doi: 10.1016/j.scitotenv.2021.147582. Epub 2021 May 13.
Soil antibiotic resistance due to animal manure application is of great concern in recent years. Little is known about the fate of antibiotic resistance genes (ARGs) in agricultural soils associated with long-term manure application. Here we used soil microcosms to investigate the dissipation of ARGs and the change of bacterial community in agricultural soil originated from a vegetable field which had received 24 years' swine manure application. Soil microcosms were conducted at different soil moistures and with or without biochar over a testing period of two years in lab. Results showed that continuous manure application induced an accumulation of ARGs in soil, wherein the dissipation of ARGs differed from those in non-manure amended soil. ARGs persisted in soils at least two years, although their abundance declined gradually. Meanwhile, soil moisture and biochar had significant impact on the fate of ARGs. ARGs dissipated faster in soil with higher moisture. Biochar amendment contributed to the maintenance of bacterial diversity. Within the two years of simulation experiment, biochar enhanced soil ARG retention as they dissipated slowly in the soil amended with biochar. Succession of microbial community may have sustained the transfer and resilience of ARGs. This study provides insight into the dissipation of antibiotic resistance genes in manure-applied agricultural soil.
近年来，由于动物粪便的施用，土壤中的抗生素抗性引起了极大的关注。对于与长期施用粪便相关的农业土壤中抗生素抗性基因（ARGs）的命运，人们知之甚少。在这里，我们使用土壤微宇宙来研究源自一个蔬菜田的农业土壤中 ARGs 的消散和细菌群落的变化，该蔬菜田已经接受了 24 年的猪粪施用。在实验室中，在两年的测试期内，在不同的土壤湿度下并在有或没有生物炭的情况下进行了土壤微宇宙实验。结果表明，连续的粪肥施用会导致土壤中 ARGs 的积累，其中 ARGs 的消散与未施粪肥的土壤不同。尽管 ARGs 的丰度逐渐下降，但 ARGs 至少在土壤中存在了两年。同时，土壤湿度和生物炭对 ARGs 的命运有显著影响。在较高湿度的土壤中，ARGs 消散得更快。生物炭的添加有助于维持细菌多样性。在两年的模拟实验中，由于生物炭的添加使土壤 ARG 保留下来，因此 ARGs 在添加生物炭的土壤中缓慢消散。微生物群落的演替可能维持了 ARGs 的转移和恢复能力。本研究深入了解了施用于农业土壤中的抗生素抗性基因的消散。
