College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China.
College of Life Science, Northeast Agricultural University, Harbin, China.
Reprod Domest Anim. 2021 Aug;56(8):1095-1103. doi: 10.1111/rda.13952. Epub 2021 May 26.
In the area of stem cell research, fusion of somatic cells into pluripotent cells such as mouse embryonic stem (ES) cells induces reprogramming of the somatic nucleus and can be used to study the effect of trans-acting factors from the pluripotent cell on the pluripotent state of somatic nucleus. As many other groups, we previously established a porcine pluripotent cell line at a low potential. Therefore, here, we performed experiments to investigate if the fusion with mouse ES cell could improve the pluripotent state of porcine pluripotent cell. Our data showed that resultant mouse-porcine interspecies fused cells are AP positive, and could be passaged up to 20 passages. Different degrees of increases in expression of porcine pluripotent genes proved that pig-origin gene network can be programmed by mouse ES. Further differentiation study also confirmed these fused cells' potential to form three germ layers. However, unexpectedly, we found that chromosome loss and aberrant (especially in porcine chromosomes) is severe after the cell fusion, implying that interspecies cell fusion may be not suitable to study porcine pluripotency without additional supportive conditions for genome stabilization.
在干细胞研究领域，体细胞与多能细胞（如小鼠胚胎干细胞（ES）细胞）融合可诱导体细胞核重编程，并可用于研究多能细胞中的转录因子对体细胞核的多能状态的影响。与许多其他研究小组一样，我们之前在低潜能下建立了一种猪多能细胞系。因此，在这里，我们进行了实验来研究与小鼠 ES 细胞融合是否可以改善猪多能细胞的多能状态。我们的数据表明，所得的鼠-猪种间融合细胞呈 AP 阳性，并且可以传代 20 代以上。猪多能基因表达水平的不同程度增加证明了猪起源的基因网络可以被小鼠 ES 编程。进一步的分化研究也证实了这些融合细胞形成三个胚层的潜力。然而，出乎意料的是，我们发现细胞融合后染色体丢失和异常（尤其是在猪染色体中）很严重，这意味着如果没有基因组稳定的额外支持条件，种间细胞融合可能不适合研究猪的多能性。
