Liu Fang, Wang Jing, Yue Yongli, Li Chen, Zhang Xuemin, Xiang Jinzhu, Wang Hanning, Li Xueling
State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot 010070, China.
Int J Mol Sci. 2023 Sep 29;24(19):14728. doi: 10.3390/ijms241914728.
The Arbas cashmere goat is a unique biological resource that plays a vital role in livestock husbandry in China. LCDM is a medium with special small molecules (consisting of human LIF, CHIR99021, (S)-(+)-dimethindene maleate, and minocycline hydrochloride) for generation pluripotent stem cells (PSCs) with bidirectional developmental potential in mice, humans, pigs, and bovines. However, there is no report on whether LCDM can support for generation of PSCs with the same ability in Arbas cashmere goats. In this study, we applied LCDM to generate goat induced PSCs (giPSCs) from goat fetal fibroblasts (GFFs) by reprogramming. The derived giPSCs exhibited stem cell morphology, expressing pluripotent markers, and could differentiate into three germ layers. Moreover, the giPSCs differentiated into the trophectoderm lineage by spontaneous and directed differentiation in vitro. The giPSCs contributed to embryonic and extraembryonic tissue in preimplantation blastocysts and postimplantation chimeric embryos. RNA-sequencing analysis showed that the giPSCs were very close to goat embryos at the blastocyst stage and giPSCs have similar properties to typical extended PSCs (EPSCs). The establishment of giPSCs with LCDM provides a new way to generate PSCs from domestic animals and lays the foundation for basic and applied research in biology and agriculture.
阿尔巴斯绒山羊是一种独特的生物资源，在中国畜牧业中发挥着至关重要的作用。LCDM是一种含有特殊小分子（由人白血病抑制因子、CHIR99021、（S）-（+）-马来酸氯苯那敏和盐酸米诺环素组成）的培养基，用于在小鼠、人类、猪和牛中生成具有双向发育潜能的多能干细胞（PSC）。然而，关于LCDM是否能支持在阿尔巴斯绒山羊中生成具有相同能力的PSC，尚无相关报道。在本研究中，我们应用LCDM通过重编程从山羊胎儿成纤维细胞（GFF）中生成山羊诱导多能干细胞（giPSC）。所获得的giPSC表现出干细胞形态，表达多能性标志物，并能分化为三个胚层。此外，giPSC在体外通过自发和定向分化分化为滋养外胚层谱系。giPSC在植入前囊胚和植入后嵌合胚胎中对胚胎和胚外组织有贡献。RNA测序分析表明，giPSC在囊胚阶段与山羊胚胎非常接近，并且giPSC具有与典型的扩展多能干细胞（EPSC）相似的特性。利用LCDM建立giPSC为从家畜中生成PSC提供了一种新方法，并为生物学和农业的基础研究和应用研究奠定了基础。
