Morch M D, Boyer J C, Haenni A L
Institut Jacques Monod, CNRS-Université Paris VII, Paris, France.
Nucleic Acids Res. 1988 Jul 11;16(13):6157-73. doi: 10.1093/nar/16.13.6157.
The complete nucleotide sequence of turnip yellow mosaic virus (TYMV) genomic RNA has been determined on a set of overlapping cDNA clones using a sequential sequencing strategy. The RNA is 6318 nucleotides long, excluding the cap structure. The genome organization deduced from the sequence confirms previous results of in vitro translation. A novel open reading frame (ORF) putatively encoding a Pro-rich and very basic 69K (K = kilodalton) protein is detected at the 5' end of the genome. It is initiated at the first AUG codon on the RNA and overlaps the major ORF that encodes the non structural 206K (previously referred to as 195K) protein of TYMV; its function is unknown. Several amino acid consensus sequences already described among plant and animal viruses are also found in the TYMV-encoded polypeptides. A comparison with other viruses whose RNA sequence is known leads to the conclusion that TYMV belongs to the "Sindbis-like" supergroup of viruses and could be related to Semliki forest virus.
采用连续测序策略,通过一组重叠的互补DNA(cDNA)克隆确定了芜菁黄花叶病毒(TYMV)基因组RNA的完整核苷酸序列。该RNA长度为6318个核苷酸,不包括帽结构。从序列推导的基因组结构证实了先前体外翻译的结果。在基因组的5'端检测到一个新的开放阅读框(ORF),推测其编码富含脯氨酸且碱性很强的69K(K =千道尔顿)蛋白。它从RNA上的第一个AUG密码子开始,与编码TYMV非结构206K(以前称为195K)蛋白的主要ORF重叠;其功能未知。在TYMV编码的多肽中还发现了一些已在植物和动物病毒中描述的氨基酸共有序列。与其他已知RNA序列的病毒进行比较得出结论,TYMV属于“辛德比斯样”病毒超群,可能与Semliki森林病毒有关。