通过芜菁黄花叶病毒基因组RNA的全核苷酸测序揭示的重叠开放阅读框。
Overlapping open reading frames revealed by complete nucleotide sequencing of turnip yellow mosaic virus genomic RNA.
作者信息
Morch M D, Boyer J C, Haenni A L
机构信息
Institut Jacques Monod, CNRS-Université Paris VII, Paris, France.
出版信息
Nucleic Acids Res. 1988 Jul 11;16(13):6157-73. doi: 10.1093/nar/16.13.6157.
Abstract
The complete nucleotide sequence of turnip yellow mosaic virus (TYMV) genomic RNA has been determined on a set of overlapping cDNA clones using a sequential sequencing strategy. The RNA is 6318 nucleotides long, excluding the cap structure. The genome organization deduced from the sequence confirms previous results of in vitro translation. A novel open reading frame (ORF) putatively encoding a Pro-rich and very basic 69K (K = kilodalton) protein is detected at the 5' end of the genome. It is initiated at the first AUG codon on the RNA and overlaps the major ORF that encodes the non structural 206K (previously referred to as 195K) protein of TYMV; its function is unknown. Several amino acid consensus sequences already described among plant and animal viruses are also found in the TYMV-encoded polypeptides. A comparison with other viruses whose RNA sequence is known leads to the conclusion that TYMV belongs to the "Sindbis-like" supergroup of viruses and could be related to Semliki forest virus.
摘要
采用连续测序策略，通过一组重叠的互补DNA（cDNA）克隆确定了芜菁黄花叶病毒（TYMV）基因组RNA的完整核苷酸序列。该RNA长度为6318个核苷酸，不包括帽结构。从序列推导的基因组结构证实了先前体外翻译的结果。在基因组的5'端检测到一个新的开放阅读框（ORF），推测其编码富含脯氨酸且碱性很强的69K（K =千道尔顿）蛋白。它从RNA上的第一个AUG密码子开始，与编码TYMV非结构206K（以前称为195K）蛋白的主要ORF重叠；其功能未知。在TYMV编码的多肽中还发现了一些已在植物和动物病毒中描述的氨基酸共有序列。与其他已知RNA序列的病毒进行比较得出结论，TYMV属于“辛德比斯样”病毒超群，可能与Semliki森林病毒有关。
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本文引用的文献
1
Post-Translational Proteolytic Cleavage of In Vitro-Synthesized Turnip Yellow Mosaic Virus RNA-Coded High-Molecular-Weight Proteins.体外合成的芜菁黄花叶病毒RNA编码的高分子量蛋白质的翻译后蛋白水解切割
J Virol. 1980 Apr;34(1):85-94. doi: 10.1128/JVI.34.1.85-94.1980.
2
In vitro transcription of infectious RNAs from full-length cDNAs of tobacco mosaic virus.从烟草花叶病毒全长 cDNA 体外转录传染性 RNA。
Proc Natl Acad Sci U S A. 1986 Jul;83(14):5043-7. doi: 10.1073/pnas.83.14.5043.
3
Nucleotide sequence at the 5' extremity of turnip yellow mosaic virus genome RNA.芜菁黄花叶病毒基因组RNA 5'末端的核苷酸序列。
Proc Natl Acad Sci U S A. 1978 Jul;75(7):3168-72. doi: 10.1073/pnas.75.7.3168.
4
The organisation and interviral homologies of genes at the 3' end of tobacco rattle virus RNA1.烟草脆裂病毒 RNA1 3' 端基因的组织和间隔同源性。
EMBO J. 1986 Feb;5(2):223-9. doi: 10.1002/j.1460-2075.1986.tb04202.x.
5
The nucleotide sequence of cowpea mosaic virus B RNA.豇豆花叶病毒 B RNA 的核苷酸序列。
EMBO J. 1983;2(12):2253-8. doi: 10.1002/j.1460-2075.1983.tb01731.x.
6
The relationship of certain tymoviruses assessed from the amino acid composition of their coat proteins.通过某些番茄病毒外壳蛋白的氨基酸组成评估它们之间的关系。
Intervirology. 1980;13(2):99-109. doi: 10.1159/000149114.
7
Polyamines stimulate suppression of amber termination codons in vitro by normal tRNAs.多胺在体外通过正常转运RNA刺激琥珀色终止密码子的抑制作用。
Eur J Biochem. 1980 Apr;105(3):445-51. doi: 10.1111/j.1432-1033.1980.tb04519.x.
8
Proteolytic maturation of the turnip-yellow-mosaic-virus polyprotein coded in vitro occurs by internal catalysis.体外编码的芜菁黄花叶病毒多聚蛋白的蛋白水解成熟通过内部催化发生。
Eur J Biochem. 1982 Oct;127(2):259-65. doi: 10.1111/j.1432-1033.1982.tb06864.x.
9
Attenuation in the control of expression of bacterial operons.细菌操纵子表达调控中的衰减作用。
Nature. 1981 Feb 26;289(5800):751-8. doi: 10.1038/289751a0.
10
"Nonrandom" DNA sequence analysis in bacteriophage M13 by the dideoxy chain-termination method.采用双脱氧链终止法对噬菌体M13进行“非随机”DNA序列分析。
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