Hu Hai-Jing, Wang Jian-Rui, Cheng Xian-Hao, Liu Yu, Zhang Xiao-Yan
Shandong Key Laboratory of Edible Mushroom Technology, School of Agriculture, Ludong University, Yantai 264025, China.
Biology (Basel). 2022 Apr 10;11(4):574. doi: 10.3390/biology11040574.
Oyster mushroom spherical virus (OMSV) is a positive-sense single-stranded RNA mycovirus which is associated with a devastating oyster mushroom die-back disease. However, little is known about its diversity, and the effects of OMSV infection on its fungal host are not well understood. In this study, we determined the nearly complete nucleotide sequence of OMSV isolated from cultivated oyster mushrooms in China. Sequence analysis suggested that the virus represents a new strain of OMSV (referred to here as OMSV-Ch). A GenBank BLAST search of the genomic sequences demonstrated that the OMSV-Ch had the highest identity (74.9%) with the OMSV from Korea (OMSV-Kr). At the amino acid-sequence level, these two strains shared 84.1% identity in putative replication protein (RP) and 94.1% identity in coat protein (CP). Phylogenetic analysis based on RP showed that OMSV-Ch clustered with OMSV-Kr, closely related to . Phylogenetic analysis based on both the RP and CP showed that OMSV had a distant clade relationship with tymoviruses, marafiviruses, and maculaviruses. We obtained the OMSV-Ch-free strain via single hyphal tip cultures combined with high-temperature treatment. Preliminary studies indicate that OMSV-Ch can significantly inhibit mycelial growth, cause malformations of the fruiting bodies, and reduce the yield of . Co-cultivation resulted in horizontal transmission of the OMSV-Ch to a virus-cured strain. The findings of our study contribute to the prevention and control of mycoviral diseases in the future.
平菇球形病毒（OMSV）是一种正义单链RNA真菌病毒，与毁灭性的平菇枯萎病有关。然而，对其多样性了解甚少，且OMSV感染对其真菌宿主的影响也尚未完全清楚。在本研究中，我们测定了从中国栽培的平菇中分离出的OMSV的近乎完整的核苷酸序列。序列分析表明，该病毒代表OMSV的一个新菌株（此处称为OMSV-Ch）。对基因组序列进行的GenBank BLAST搜索显示，OMSV-Ch与来自韩国的OMSV（OMSV-Kr）具有最高的同一性（74.9%）。在氨基酸序列水平上，这两个菌株在假定的复制蛋白（RP）中具有84.1%的同一性，在衣壳蛋白（CP）中具有94.1%的同一性。基于RP的系统发育分析表明，OMSV-Ch与OMSV-Kr聚类，与……密切相关。基于RP和CP的系统发育分析表明，OMSV与番茄病毒、马拉病毒和黄斑病毒具有较远的进化枝关系。我们通过单菌丝尖端培养结合高温处理获得了不含OMSV-Ch的菌株。初步研究表明，OMSV-Ch可显著抑制菌丝生长，导致子实体畸形，并降低……的产量。共培养导致OMSV-Ch水平传播至病毒治愈菌株。我们的研究结果有助于未来对真菌病毒病的预防和控制。
