Wang Rui-Qi, Chen Gang, Chen Sun-Ni, Zhu Hong-Lin, Xiong Wen-Neng, Xu Mao, Jian Su-Ping
State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing East Road, Qingshanhu District, Nanchang, 330047 Jiangxi, China.
State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing East Road, Qingshanhu District, Nanchang, 330047 Jiangxi, China.
J Biosci Bioeng. 2021 Aug;132(2):148-153. doi: 10.1016/j.jbiosc.2021.04.003. Epub 2021 May 11.
Neurospora crassa has been generally recognized as a safe organism and possesses a remarkable ability to produce yellow-to-orange carotenoids. The present work mainly explored the potential mechanism of exogenous oleic acid on promoting lycopene production in N. crassa. Carbon flux was conducively channelized into the mevalonate metabolic pathway to synthesize more lycopene, associating with the increased levels of acetyl-CoA, NADPH and factors related to the mevalonate pathway. Additionally, exogenous oleic acid boosted the intracellular triacylglycerol production through de novo and ex novo fatty acid synthesis pathways, which contributed to improving the accumulation of lycopene via lipid bodies. Further, the regulated fatty acid profile also enhanced the storage capacity of lipid bodies. Consequently, this study provided an effective strategy to enhance the lycopene production in N. crassa by adding oleic acid to the culture medium and elucidated an extraordinary insight into the potential mechanism.
粗糙脉孢菌通常被认为是一种安全的生物体，并且具有产生黄色至橙色类胡萝卜素的显著能力。本研究主要探讨了外源油酸促进粗糙脉孢菌中番茄红素产生的潜在机制。碳通量被有利地引导到甲羟戊酸代谢途径中以合成更多的番茄红素，这与乙酰辅酶A、NADPH以及与甲羟戊酸途径相关的因子水平增加有关。此外，外源油酸通过从头合成和重新合成脂肪酸途径促进细胞内三酰甘油的产生，这有助于通过脂质体提高番茄红素的积累。此外，调节后的脂肪酸谱也增强了脂质体的储存能力。因此，本研究提供了一种通过向培养基中添加油酸来提高粗糙脉孢菌中番茄红素产量的有效策略，并阐明了潜在机制的非凡见解。
