Department of Biochemistry, College of Health Sciences, School of Medicine, Addis Ababa University, Addis Ababa, Ethiopia.
Department of Surgery, College of Health Sciences, School of Medicine, Addis Ababa University, Addis Ababa, Ethiopia.
Oxid Med Cell Longev. 2018 Mar 26;2018:6039453. doi: 10.1155/2018/6039453. eCollection 2018.
The exact cause of breast cancer is unknown; it is a multifactorial disease. It is the most diagnosed and the second killer cancer among women. Breast cancer can be originated from tissues of breast or secondary from other organs via metastasis. Generally, cancer cells show aberrant metabolism and oxidative stress when compared to noncancerous tissues of breast cancer patients. The current study aims at evaluating glutamate and glucose metabolism through GDH and LDH enzyme activities, oxidant, and antioxidative status among breast cancer patients attending referral hospitals of Addis Ababa, Ethiopia. . Catalytic activities of glutamate dehydrogenase, lactate dehydrogenase, and oxidative stress index were significantly increased in both serum (4.2 mU/ml, 78.6 mU/ml, and 3.3 : 1, resp.) and cancerous tissues (1.4 mU/ml, 111.7 mU/ml, and 2.15 : 1, resp.) of breast cancer patients as compared to those in serum of control group (3.15 mU/ml, 30.4 mU/ml, and 2.05 : 1, resp.) and noncancerous tissues of breast cancer patients (0.92 mU/ml, 70.5 mU/ml, and 1.1 : 1, resp.) ( ≤ 0.05). Correspondingly, ratios of reduced to oxidized glutathione were significantly decreased in both serum (20 : 1) and cancerous tissues (23.5 : 1) of breast cancer patients when compared to those in serum of control group (104.5 : 1) and noncancerous tissues of breast cancer patients (70.9 : 1) ( ≤ 0.05). . Catalytic activities of GDH and LDH, ratios of GSH to GSSG, and concentration of TOS among breast cancer patients were significantly higher than were those among control group and noncancerous tissues of breast cancer patients, while TAC of breast cancer patients is significantly lower than that of control group and normal tissues of breast cancer patients.
乳腺癌的确切病因尚不清楚；它是一种多因素疾病。它是女性中诊断最多的第二大癌症杀手。乳腺癌可以起源于乳房组织，也可以通过转移来自其他器官。一般来说，与乳腺癌患者的非癌组织相比，癌细胞表现出异常代谢和氧化应激。本研究旨在评估谷氨酸和葡萄糖代谢通过 GDH 和 LDH 酶活性、氧化剂和抗氧化状态在埃塞俄比亚亚的斯亚贝巴转诊医院就诊的乳腺癌患者中。与对照组血清（3.15 mU/ml、30.4 mU/ml 和 2.05：1）和乳腺癌患者非癌组织（0.92 mU/ml、70.5 mU/ml 和 1.1：1）相比，乳腺癌患者血清（4.2 mU/ml、78.6 mU/ml 和 3.3：1，分别）和癌组织（1.4 mU/ml、111.7 mU/ml 和 2.15：1，分别）中谷氨酸脱氢酶、乳酸脱氢酶的催化活性和氧化应激指数显著增加（≤ 0.05）。相应地，与对照组血清（104.5：1）和乳腺癌患者非癌组织（70.9：1）相比，乳腺癌患者血清（20：1）和癌组织（23.5：1）中还原型谷胱甘肽与氧化型谷胱甘肽的比值显著降低（≤ 0.05）。与对照组和乳腺癌患者非癌组织相比，乳腺癌患者的 GDH 和 LDH 催化活性、GSH 与 GSSG 的比值和 TOS 浓度显著升高，而乳腺癌患者的 TAC 显著降低。
