大肠杆菌染色体上分散的甲硫氨酸基因的物理图谱
Physical mapping of the scattered methionine genes on the Escherichia coli chromosome.
作者信息
Old I G, Saint Girons I, Richaud C
机构信息
Unité de Bactériologie Moléculaire et Médicale, Institut Pasteur, Paris, France.
出版信息
J Bacteriol. 1993 Jun;175(11):3689-91. doi: 10.1128/jb.175.11.3689-3691.1993.
Abstract
Methionine is an important amino acid which acts not only as a substrate for protein elongation but also as the initiator of protein synthesis. The genes of the met regulon, which consists of 10 biosynthetic genes (metA, metB, metC, metE, metF, metH, metK, metL, metQ, and metX), two regulatory genes (metJ and metR), and the methionyl tRNA synthetase gene (metG), are scattered throughout the chromosome. The only linked genes are metK and metX at 63.6 min, metE and metR at 86.3 min, and the metJBLF gene cluster at 89 min. metBL form the only met operon.
摘要
甲硫氨酸是一种重要的氨基酸，它不仅作为蛋白质延伸的底物，还作为蛋白质合成的起始物。甲硫氨酸调节子的基因散布于整个染色体，该调节子由10个生物合成基因（metA、metB、metC、metE、metF、metH、metK、metL、metQ和metX）、两个调控基因（metJ和metR）以及甲硫氨酰tRNA合成酶基因（metG）组成。唯一相连的基因是位于63.6分钟处的metK和metX、位于86.3分钟处的metE和metR，以及位于89分钟处的metJBLF基因簇。metB和metL构成唯一的met操纵子。
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