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猪流行性腹泻病毒抗原与免疫球蛋白G的多聚Fc融合并在ΔXT/FT植物中表达后的全身及口服免疫原性

Systemic and Oral Immunogenicity of Porcine Epidemic Diarrhea Virus Antigen Fused to Poly-Fc of Immunoglobulin G and Expressed in ΔXT/FT Plants.

作者信息

Tien Nguyen-Quang-Duc, Yang Moon-Sik, Jang Yong-Suk, Kwon Tae-Ho, Reljic Rajko, Kim Mi-Young

机构信息

Department of Molecular Biology, Jeonbuk National University, Jeonju-Si, South Korea.

University of Sciences, Hue University, Hue City, Viet Nam.

出版信息

Front Pharmacol. 2021 Apr 30;12:653064. doi: 10.3389/fphar.2021.653064. eCollection 2021.

DOI:10.3389/fphar.2021.653064
PMID:33995068
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8120289/
Abstract

Porcine epidemic diarrhea virus (PEDV), a member of the Coronaviridae family has become increasingly probelmatic in the pig farming industry. Currently, there are no effective, globally applicable vaccines against PEDV. Here, we tested a recombinant PEDV vaccine candidate based on the expression of the core neutralising epitope (COE) of PEDV conjugated to polymeric immunoglobulin G scaffold (PIGS) in glycoengineered plants. The biological activity of COE-PIGS was demonstrated by binding to C1q component of the complement system, as well as the surface of antigen-presenting cells (APCs) The recombinant COE-PIGS induced humoral and cellular immune responses specific for PEDV after both systemic and mucosal vaccination. Altogether, the data indicated that PEDV antigen fusion to poly-Fc could be a promising vaccine platform against respiratory PEDV infection.

摘要

猪流行性腹泻病毒（PEDV）是冠状病毒科的成员，在养猪业中已变得越来越成问题。目前，尚无针对PEDV的有效、全球适用的疫苗。在此，我们测试了一种重组PEDV候选疫苗，该疫苗基于在糖工程植物中表达与聚合免疫球蛋白G支架（PIGS）缀合的PEDV核心中和表位（COE）。COE-PIGS的生物活性通过与补体系统的C1q成分以及抗原呈递细胞（APC）表面结合来证明。重组COE-PIGS在全身和黏膜接种后均诱导了针对PEDV的体液和细胞免疫反应。总之，数据表明PEDV抗原与多聚Fc融合可能是对抗呼吸道PEDV感染的一个有前景的疫苗平台。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75c5/8120289/2c2fe92af857/fphar-12-653064-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75c5/8120289/6cddf87ba8f1/fphar-12-653064-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75c5/8120289/6a4e12012319/fphar-12-653064-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75c5/8120289/2c2fe92af857/fphar-12-653064-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75c5/8120289/6cddf87ba8f1/fphar-12-653064-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75c5/8120289/83e0aa0f3ce2/fphar-12-653064-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75c5/8120289/baf5e6939f10/fphar-12-653064-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75c5/8120289/6a4e12012319/fphar-12-653064-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75c5/8120289/2c2fe92af857/fphar-12-653064-g005.jpg

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