Department of Pharmaceutical Microbiology, Medical University of Lublin, 1 Chodźki St., 20-093 Lublin, Poland.
Department of Biology and Genetics, Medical University of Lublin, 4a Chodźki St., 20-093 Lublin, Poland.
Int J Mol Sci. 2022 Dec 26;24(1):368. doi: 10.3390/ijms24010368.
The filamentation ability of represents one of the main virulence factors allowing for host tissue penetration and biofilm formation. The aim of this paper was to study the genetic background of the hyperfilamentous biofilm development in vitro in isolated from the oral cavity of a lung cancer patient. Analyzed isolates (CA1, CA2, CA3) were chosen based on their different structures of mature biofilm. The CA3 isolate possessing hyperfilamentation properties and forming high biofilm was compared with CA1 and CA2 isolates exhibiting low or average biofilm-forming ability, respectively. The detailed biofilm organization was studied with the use of confocal scanning laser microscopy. The whole transcriptome analysis was conducted during three stages of biofilm development (24 h, 48 h, 72 h). In contrast to CA1 and/or CA2 isolate, the CA3 isolate was characterized by a significant upregulation of genes encoding for cell wall proteins (HWP1, PGA13, PGA44, ALS3) and candidalysin (ECE1), as well as being involved in iron metabolism (FRE1, ALS3), sulfur metabolism (HAL21), the degradation of aromatic compounds (HQD2), and membrane transport (DIP5, PHO89, TNA1). In contrast, some genes (SCW11, FGR41, RBE1) in the CA3 were downregulated. We also observed the overexpression of a few genes over time-mainly FRE1, ATX1, CSA2 involved in iron metabolism. This is the first insight into the potential function of multiple genes in the hyperfilamentous biofilm formation in , primarily isolated from host tissue, which may have an important clinical impact on cancer patients. Moreover, the presented data can lay the foundation for further research on novel pathogen-specific targets for antifungal drugs.
菌毛形成能力是 代表主要毒力因子之一，可实现宿主组织穿透和生物膜形成。本文旨在研究从肺癌患者口腔中分离出的 体外超丝状生物膜形成的遗传背景。选择了具有不同成熟生物膜结构的 3 个分析分离株（CA1、CA2、CA3）。与分别表现出低或平均生物膜形成能力的 CA1 和 CA2 分离株相比，选择具有超丝状形成特性并形成高生物膜的 CA3 分离株进行比较。利用共聚焦扫描激光显微镜研究详细的生物膜组织。在生物膜发育的三个阶段（24 h、48 h、72 h）进行全转录组分析。与 CA1 和/或 CA2 分离株相比，CA3 分离株的细胞壁蛋白（HWP1、PGA13、PGA44、ALS3）和念珠菌素（ECE1）编码基因的表达显著上调，同时还参与铁代谢（FRE1、ALS3）、硫代谢（HAL21）、芳香族化合物降解（HQD2）和膜转运（DIP5、PHO89、TNA1）。相比之下，CA3 中的一些基因（SCW11、FGR41、RBE1）下调。我们还观察到一些基因随着时间的推移过度表达-主要是 FRE1、ATX1、CSA2，它们参与铁代谢。这是首次深入了解 中多个基因在主要从宿主组织中分离的超丝状生物膜形成中的潜在功能，这可能对癌症患者具有重要的临床影响。此外，所提供的数据可以为进一步研究针对新型病原体的抗真菌药物的特定靶标奠定基础。
