Petrova Natalia, Nazipova Alsu, Gorshkov Oleg, Mokshina Natalia, Patova Olga, Gorshkova Tatyana
Laboratory of Plant Glycobiology, Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS, Kazan, Russia.
Laboratory of Plant Cell Growth Mechanisms, Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS, Kazan, Russia.
Front Plant Sci. 2021 Apr 26;12:634594. doi: 10.3389/fpls.2021.634594. eCollection 2021.
The genomes of higher plants encode a variety of proteins with lectin domains that are able to specifically recognize certain carbohydrates. Plants are enriched in a variety of potentially complementary glycans, many of which are located in the cell wall. We performed a genome-wide search for flax proteins with lectin domains and compared the expression of the encoding genes in different stem tissues that have distinct cell wall types with different sets of major polysaccharides. Over 400 genes encoding proteins with lectin domains that belong to different families were revealed in the flax genome; three quarters of these genes were expressed in stem tissues. Hierarchical clustering of the data for all expressed lectins grouped the analyzed samples according to their characteristic cell wall type. Most lectins differentially expressed in tissues with primary, secondary, and tertiary cell walls were predicted to localize at the plasma membrane or cell wall. These lectins were from different families and had various architectural types. Three out of four flax genes for proteins with jacalin-like domains were highly upregulated in bast fibers at the stage of tertiary cell wall deposition. The dynamic changes in transcript level of many genes for lectins from various families were detected in stem tissue over the course of gravitropic response induced by plant gravistimulation. The data obtained in this study indicate a large number of lectin-mediated events in plants and provide insight into the proteins that take part in tissue specialization and reaction to abiotic stress.
高等植物的基因组编码多种具有凝集素结构域的蛋白质，这些蛋白质能够特异性识别某些碳水化合物。植物富含多种潜在互补的聚糖，其中许多位于细胞壁中。我们对亚麻中具有凝集素结构域的蛋白质进行了全基因组搜索，并比较了编码基因在不同茎组织中的表达情况，这些组织具有不同类型的细胞壁以及不同的主要多糖组合。在亚麻基因组中发现了超过400个编码属于不同家族的具有凝集素结构域的蛋白质的基因；其中四分之三的基因在茎组织中表达。对所有表达的凝集素数据进行层次聚类，根据其特征性细胞壁类型对分析样本进行了分组。大多数在具有初生、次生和三生细胞壁的组织中差异表达的凝集素预计定位于质膜或细胞壁。这些凝集素来自不同家族，具有不同的结构类型。四个具有jacalin样结构域的蛋白质的亚麻基因中有三个在三生细胞壁沉积阶段的韧皮纤维中高度上调。在植物重力刺激诱导的重力反应过程中，在茎组织中检测到来自不同家族的许多凝集素基因转录水平的动态变化。本研究获得的数据表明植物中存在大量凝集素介导的事件，并为参与组织特化和对非生物胁迫反应的蛋白质提供了见解。
