Zhou Li, Yu Xiaoding, Wang Donghao, Li Lin, Zhou Wen, Zhang Qian, Wang Xinrui, Ye Sumin, Wang Zhezhi
National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi, China.
PeerJ. 2021 May 6;9:e11345. doi: 10.7717/peerj.11345. eCollection 2021.
Heat shock transcription factors (HSFs) are critical regulators of plant responses to various abiotic and biotic stresses, including high temperature stress. HSFs are involved in regulating the expression of heat shock proteins (HSPs) by binding with heat stress elements (HSEs) to defend against high-temperature stress. The genome was recently fully sequenced; this provides a valuable resource for genetic and functional analysis. In this study, 23 putative genes were identified and divided into three groups (A, B, and C) based on phylogeny and structural features. Gene structure and conserved motif analyses were performed on members; the DNA-binding domain (DBD), hydrophobic heptad repeat (HR-A/B), and exon-intron boundaries exhibited specific phylogenetic relationships. In addition, the presence of various -acting elements in the promoter regions of underscored their regulatory function in abiotic stress responses. RT-qPCR analyses showed that most genes were expressed in response to heat conditions, suggesting that HpHSFs play potential roles in the heat stress resistance pathway. Our findings are advantageous for the analysis and research of the function of HpHSFs in high temperature stress tolerance in .
热激转录因子（HSFs）是植物对包括高温胁迫在内的各种非生物和生物胁迫响应的关键调节因子。HSFs通过与热应激元件（HSEs）结合参与调节热激蛋白（HSPs）的表达，以抵御高温胁迫。该基因组最近已完成全序列测定；这为遗传和功能分析提供了宝贵资源。在本研究中，鉴定出23个假定基因，并根据系统发育和结构特征将其分为三组（A、B和C）。对成员进行了基因结构和保守基序分析；DNA结合结构域（DBD）、疏水七肽重复序列（HR-A/B）和外显子-内含子边界呈现出特定的系统发育关系。此外，启动子区域中各种顺式作用元件的存在强调了它们在非生物胁迫响应中的调节功能。RT-qPCR分析表明，大多数基因在热条件下表达，这表明HpHSFs在热胁迫抗性途径中发挥潜在作用。我们的研究结果有利于分析和研究HpHSFs在[植物名称]高温胁迫耐受性中的功能。
