Chidambaranathan Parameswaran, Jagannadham Prasanth Tej Kumar, Satheesh Viswanathan, Kohli Deshika, Basavarajappa Santosh Halasabala, Chellapilla Bharadwaj, Kumar Jitendra, Jain Pradeep Kumar, Srinivasan R
National Research Centre on Plant Biotechnology, New Delhi, India.
Indian Agricultural Research Institute, New Delhi, India.
J Plant Res. 2018 May;131(3):525-542. doi: 10.1007/s10265-017-0948-y. Epub 2017 May 4.
The heat stress transcription factors (Hsfs) play a prominent role in thermotolerance and eliciting the heat stress response in plants. Identification and expression analysis of Hsfs gene family members in chickpea would provide valuable information on heat stress responsive Hsfs. A genome-wide analysis of Hsfs gene family resulted in the identification of 22 Hsf genes in chickpea in both desi and kabuli genome. Phylogenetic analysis distinctly separated 12 A, 9 B, and 1 C class Hsfs, respectively. An analysis of cis-regulatory elements in the upstream region of the genes identified many stress responsive elements such as heat stress elements (HSE), abscisic acid responsive element (ABRE) etc. In silico expression analysis showed nine and three Hsfs were also expressed in drought and salinity stresses, respectively. Q-PCR expression analysis of Hsfs under heat stress at pod development and at 15 days old seedling stage showed that CarHsfA2, A6, and B2 were significantly upregulated in both the stages of crop growth and other four Hsfs (CarHsfA2, A6a, A6c, B2a) showed early transcriptional upregulation for heat stress at seedling stage of chickpea. These subclasses of Hsfs identified in this study can be further evaluated as candidate genes in the characterization of heat stress response in chickpea.
热应激转录因子（Hsfs）在植物的耐热性及引发热应激反应中发挥着重要作用。鹰嘴豆中Hsfs基因家族成员的鉴定与表达分析将为热应激响应Hsfs提供有价值的信息。对Hsfs基因家族进行全基因组分析，在鹰嘴豆的迪西和卡布利基因组中均鉴定出22个Hsfs基因。系统发育分析分别清晰地分离出12个A类、9个B类和1个C类Hsfs。对基因上游区域的顺式调控元件分析鉴定出许多应激响应元件，如热应激元件（HSE）、脱落酸响应元件（ABRE）等。电子表达分析表明，分别有9个和3个Hsfs也在干旱和盐胁迫中表达。对鹰嘴豆荚发育阶段和15日龄幼苗期热应激下Hsfs的Q-PCR表达分析表明，CarHsfA2、A6和B2在作物生长的两个阶段均显著上调，其他4个Hsfs（CarHsfA2、A6a、A6c、B2a）在鹰嘴豆幼苗期对热应激表现出早期转录上调。本研究中鉴定出的这些Hsfs亚类可进一步作为候选基因，用于鹰嘴豆热应激反应特性的研究。
