Asciak Rachelle, Kanellakis Nikolaos I, Yao Xuan, Abd Hamid Megat, Mercer Rachel M, Hassan Maged, Bedawi Eihab O, Dobson Melissa, Fsadni Peter, Montefort Stephen, Dong Tao, Rahman Najib M, Psallidas Ioannis
Oxford Centre for Respiratory Medicine, Churchill Hospital, Oxford University Hospitals NHS Foundation Trust, Oxford, United Kingdom.
Laboratory of Pleural and Lung Cancer Translational Research, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom.
Front Oncol. 2021 Apr 28;11:658395. doi: 10.3389/fonc.2021.658395. eCollection 2021.
Patients with malignant pleural mesothelioma (MPM) or pleural metastases often present with malignant pleural effusion (MPE). This study aimed to analyze the effect of pleural fluid on cancer cells.
Established patient-derived cancer cell cultures derived from MPE (MPM, breast carcinoma, lung adenocarcinoma) were seeded in 100% pleural fluid (exudate MPM MPE, transudate MPE, non-MPE transudate fluid) and proliferation was monitored. In addition, the establishment of new MPM cell cultures, derived from MPE specimens, was attempted by seeding the cells in 100% MPE fluid.
All established cancer cell cultures proliferated with similar growth rates in the different types of pleural fluid. Primary MPM cell culture success was similar with MPE fluid as with full culture medium.
Pleural fluid alone is adequate for cancer cell proliferation , regardless of the source of pleural fluid. These results support the hypothesis that pleural fluid has important pro-growth biological properties, but the mechanisms for this effect are unclear and likely not malignant effusion specific.
恶性胸膜间皮瘤（MPM）或胸膜转移患者常伴有恶性胸腔积液（MPE）。本研究旨在分析胸腔积液对癌细胞的影响。
将源自MPE（MPM、乳腺癌、肺腺癌）的已建立的患者来源癌细胞培养物接种于100%胸腔积液（渗出性MPM MPE、漏出性MPE、非MPE漏出液）中，并监测其增殖情况。此外，尝试通过将细胞接种于100% MPE液中，从MPE标本中建立新的MPM细胞培养物。
所有已建立好的癌细胞培养物在不同类型的胸腔积液中以相似的生长速率增殖。原发性MPM细胞培养成功率在MPE液中与在完全培养基中相似。
无论胸腔积液来源如何，仅胸腔积液就足以支持癌细胞增殖。这些结果支持了胸腔积液具有重要的促生长生物学特性这一假说，但这种作用的机制尚不清楚，且可能并非恶性积液所特有。
