Oxford Centre for Respiratory Medicine, Churchill Hospital, Oxford University Hospitals NHS Foundation Trust, Oxford, United Kingdom
Laboratory of Pleural and Lung Cancer Translational Research, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom.
Thorax. 2020 Nov;75(11):1004-1008. doi: 10.1136/thoraxjnl-2020-215027. Epub 2020 Sep 17.
Malignant pleural mesothelioma (MPM) is an aggressive cancer, associated with poor prognosis. We assessed the feasibility of patient-derived cell cultures to serve as an ex vivo model of MPM. Patient-derived MPM cell cultures (n=16) exhibited stemness features and reflected intratumour and interpatient heterogeneity. A subset of the cells were subjected to high-throughput drug screening and coculture assays with cancer-specific cytotoxic T cells and showed diverse responses. Some of the biphasic MPM cells were capable of processing and presenting the neoantigen SSX-2 endogenously. In conclusion, patient-derived MPM cell cultures are a promising and faithful ex vivo model of MPM.
恶性胸膜间皮瘤（MPM）是一种侵袭性癌症，预后不良。我们评估了患者来源的细胞培养物作为 MPM 的体外模型的可行性。患者来源的 MPM 细胞培养物（n=16）表现出干细胞特征，并反映了肿瘤内和患者间的异质性。一小部分细胞进行了高通量药物筛选，并与具有肿瘤特异性细胞毒性 T 细胞进行共培养试验，显示出不同的反应。一些双相 MPM 细胞能够内源性加工和呈递新抗原 SSX-2。总之，患者来源的 MPM 细胞培养物是一种有前途的、忠实的 MPM 体外模型。
