Li Xiaoting, Liu Haibin, Cao Sai, Cheng Ping, Li Fulei, Ishfaq Muhammad, Sun Jichao, Zhang Xiuying
Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Northeast Agricultural University, Harbin, China.
Department of Basic Veterinary Science, College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
Front Vet Sci. 2021 Apr 30;8:614651. doi: 10.3389/fvets.2021.614651. eCollection 2021.
Antibiotics play an essential role in the treatment and prevention of diseases in pig farms. However, the irrational use of antibiotics leads to the emergence of multi-drug resistance of bacteria, which poses a critical threat to the efficacy of antibiotic treatments. Therefore, the study is designed to analyze the drug resistance of pathogenic isolated from large-scale pig farms in East China, which provides a theoretical basis for precisely targeted clinical drugs in swine farms. The pathogenic were isolated and identified from clinical samples of swine farms, and the drug resistance of pathogenic was detected by antimicrobial susceptibility test (AST) and minimum inhibitory concentration test (MIC). Moreover, the prevalence of plasmid-mediated β-lactam resistance genes was analyzed by PCR. A total of 67 pathogenic were isolated from 152 samples collected from 20 large-scale pig farms in East China. All isolated pathogenic are associated with severe drug resistance. Moreover, 70% of isolated pathogenic is resistant to more than four antibiotics. Besides, there were 19 serotypes including O2, O4, O5, O6, O14, O26, O38, O42, O49, O57, O92, O93, O95, O101, O121, O131, O143, O158, and O161, of which the O4 and O92 serotype were the main serotypes in swine farms. The main extended-spectrum beta-lactamases (ESBLs)-encoding genes in East China were , , and by the detection of the ESBLs encoding genes of porcine pathogenic . The conjugation assays showed that a total of 30 transconjugants were obtained by conjugation, which indicated that drug resistance genes could be transmitted horizontally through conjugative plasmids. The isolated pathogenic were all multi-drug resistant, and especially O4 and O92 were the main serotypes. The β-lactam resistance genes were prevalent in large-scale pig farms in East China, which provided a theoretical basis for the prevention and control of pig-derived pathogenic in the future.
抗生素在猪场疾病的治疗和预防中发挥着重要作用。然而，抗生素的不合理使用导致细菌出现多重耐药性，这对抗生素治疗的疗效构成了严重威胁。因此，本研究旨在分析华东地区大型猪场分离出的病原菌的耐药性，为猪场精准靶向临床用药提供理论依据。从猪场临床样本中分离并鉴定病原菌，通过药敏试验（AST）和最低抑菌浓度试验（MIC）检测病原菌的耐药性。此外，采用PCR分析质粒介导的β-内酰胺耐药基因的流行情况。从华东地区20个大型猪场采集的152份样本中共分离出67株病原菌。所有分离出的病原菌都具有严重的耐药性。此外，70%的分离病原菌对四种以上抗生素耐药。此外，共有19种血清型，包括O2、O4、O5、O6、O14、O26、O38、O42、O49、O57、O92、O93、O95、O101、O121、O131、O143、O158和O161，其中O4和O92血清型是猪场的主要血清型。通过检测猪病原菌的ESBLs编码基因，华东地区主要的超广谱β-内酰胺酶（ESBLs）编码基因是 、 和 。接合试验表明，通过接合共获得30个接合子，这表明耐药基因可通过接合性质粒水平转移。分离出的病原菌均具有多重耐药性，尤其是O4和O92是主要血清型。β-内酰胺耐药基因在华东地区大型猪场中普遍存在，为今后猪源病原菌的防控提供了理论依据。
